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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM beta Actin Antibody, mAb, Mouse

THETM beta Actin Antibody, mAb, Mouse

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Lane 1: 20 µg Hela cell lysate
Lane 2: 20 µg sp2/0 cell lysate
Lane 3: 20 µg goat muscle lysate
Lane 4: 20 µg rabbit muscle lysate
Lane 5: 20 µg chicken muscle lysate
Lane 6: 20 µg CHO cell lysate
Lane 7: 20 µg fish muscle lysate

Primary antibody: 1 µg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000)

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

Synonyms cytoplasmic 1 antibody;
Description β-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to ß-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A or G affinity column
Key Features GenScript THETM beta Actin Antibody, mAb, Mouse has high sensitivity, low background in WB compared with antibody on the market.

Subclass IgG2a, k
Clone ID 2D1D10
Concentration 40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
Reconstitution Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
Specificity THETM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. It has not yet been tested in other species.
Species Reactivity Mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse
 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Lane 1: 20 µg Hela cell lysate
Lane 2: 20 µg sp2/0 cell lysate
Lane 3: 20 µg goat muscle lysate
Lane 4: 20 µg rabbit muscle lysate
Lane 5: 20 µg chicken muscle lysate
Lane 6: 20 µg CHO cell lysate
Lane 7: 20 µg fish muscle lysate

Primary antibody: 1 µg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000)

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse
 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse
 THE<sup>TM</sup> Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Cat. No.
Product Name
Size
Price
Quantity
A00702-100 THETM beta Actin Antibody, mAb, Mouse
100 ug
$175.00
A00702-40 THETM beta Actin Antibody, mAb, Mouse
40 ug
$69.00
A00702-200 THETM beta Actin Antibody, mAb, Mouse
200 ug
$311.00
A00730-200 THETM beta Actin Antibody [HRP], mAb, Mouse
200 ug
$333.00
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 ug
$99.00
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 ug
$349.00
A01546 THETM beta Actin Antibody [Biotin], mAb, Mouse
100 ug
$180.00
 
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Palavicini JP.,et al. RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer’s brain synaptosomes.Cell Death Dis.2013Jun;4:e667

Shen Y.,et al. Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles.Nucleic Acid Ther.2013Apr;23(2):95-108

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Hayes CD.,et al. Chronic Cladribine Administration Increases Amyloid Beta Peptide Generation and Plaque Burden in Mice.PLoS One.2012Otc;7(10):e45841

Ke H.,et al. CYLD Inhibits Melanoma Growth and Progression through Suppression of the JNK/AP-1 and β1-Integrin Signaling Pathways.J Invest Dermatol.2013Jan;133(1):221-9

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Cat. No. A00702
Size
Price
40 ug $69.00
200 ug $311.00
100 ug $175.00
24 hour
 
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