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Catalog Products » Antibodies » Epitope Tag Antibodies » SRT-tag Antibody, pAb, Rabbit

SRT-tag Antibody, pAb, Rabbit

SRT-tag Antibody, PAb, Rabbit

Lane 1-2: SRT-tag fusion protein in E. coli cell lysate (≈ 52 kDa)
Lane 3: Negative E. coli cell lysate
Primary Antibody: 1 µg/ml Rabbit Anti-SRT-tag Polyclonal Antibody (GenScript, A00725)
Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SRT-tag Antibody, PAb, Rabbit

Loading: SRT-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 µg/ml Rabbit Anti-SRT-tag Polyclonal Antibody (GenScript, A00725)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

SRT-tag Antibody, PAb, Rabbit

Western blot analysis of SRT tagged fusion proteins expressed in E. coli cell lysate using SRT-tag Antibody (GenScript, A00725, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-SRT-tag pAb;
Description Well-characterized antibodies for epitope tags consisting of short sequences are often used in the study of protein expression in various systems. The SRT-tag is a peptide epitope comprised of 10 amino acids derived from the crystalline surface layer protein of Rickettsia typhi1.
GenScript Rabbit Anti-SRT-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic SRT tag peptide conjugated to KLH.
GenScript Rabbit Anti-SRT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Rabbit anti-SRT tag is a synthetic SRT tag peptide with the sequence TFIGAIATDT, conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes SRT tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

SRT-tag Antibody, PAb, Rabbit
SRT-tag Antibody, PAb, Rabbit

Lane 1-2: SRT-tag fusion protein in E. coli cell lysate (≈ 52 kDa)
Lane 3: Negative E. coli cell lysate
Primary Antibody: 1 µg/ml Rabbit Anti-SRT-tag Polyclonal Antibody (GenScript, A00725)
Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SRT-tag Antibody, PAb, Rabbit
SRT-tag Antibody, PAb, Rabbit

Loading: SRT-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 µg/ml Rabbit Anti-SRT-tag Polyclonal Antibody (GenScript, A00725)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

SRT-tag Antibody, PAb, Rabbit
SRT-tag Antibody, PAb, Rabbit

Western blot analysis of SRT tagged fusion proteins expressed in E. coli cell lysate using SRT-tag Antibody (GenScript, A00725, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A00725
Size
Price
40 ug $99.00
24 hour
 
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