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Catalog Products » Other Products » THETM beta Actin Antibody [HRP], mAb, Mouse

THETM beta Actin Antibody [HRP], mAb, Mouse

Synonyms THETM beta Actin Antibody [HRP], mAb, Mouse;
Description GenScript THETM beta Actin Antibody [HRP], mAb, Mouse is HRP conjugated THETM beta Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of ß-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein G affinity column

Subclass IgG2a
Clone ID 2D1D10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM beta Actin Antibody [HRP], mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

 THE<sup>TM</sup> Beta Actin Antibody [HRP], MAb, Mouse
 THE<sup>TM</sup> Beta Actin Antibody [HRP], MAb, Mouse

Detection antibody: 1 μg/ml THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) (GenScript, A00730)

The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500).


Cat. No.
Product Name
Size
Price
Quantity
A00702-100 THETM beta Actin Antibody, mAb, Mouse
100 ug
$175.00
A00702-40 THETM beta Actin Antibody, mAb, Mouse
40 ug
$69.00
A00702-200 THETM beta Actin Antibody, mAb, Mouse
200 ug
$311.00
A00730-200 THETM beta Actin Antibody [HRP], mAb, Mouse
200 ug
$333.00
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 ug
$99.00
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 ug
$349.00
A01546 THETM beta Actin Antibody [Biotin], mAb, Mouse
100 ug
$180.00
 
Ferreira JP.,et al. Optimization of oncogene expression through intra‐population competitionBiotechnol J.2013Jul;

Luisa F. Jiménez-Soto.,et al. Dynamics of the Cag‐Type IV Secretion System of Helicobacter pylori as studied by bacterial co‐infections.Cell Microbiol.2013Jul;

Sokolowska M.,et al. Low molecular weight hyaluronan activates cytosolic phospholipase A2α and eicosanoid production in monocytes and macrophages.J Biol Chem.2013Dec;

Berry WL.,et al. Oncogenic features of the JMJD2A histone demethylase in breast cancer.Int J Oncol.2012Nov;41(5):1701-6

Chen J.,et al. Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury.Kidney Int.2012Dec;82(11):1195-207

Tsukamoto S.,et al. Functional analysis of lysosomes during mouse preimplantation embryo development.J Reprod Dev.2012Otc;59(1):22-29

Yang J.,et al. Establishment of transgenic mice carrying the gene for farnesyl pyrophosphate synthase.Biotechnol Lett.2011Apr;33(4):687-692

Hahmann C.,et al. A Predictive Nuclear Translocation Assay for Spliced X-Box–Binding Protein 1 Identifies Compounds with Known Organ Toxicities.Assay Drug Dev Technol.2011Feb;9(1):79-87

Lee I, et al. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proc Natl Acad Sci U S A. 2014Jan.;

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Cat. No. A00730
Size
Price
200 ug $333.00
100 ug $180.00
 
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