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Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » THETM beta Actin Antibody [HRP], mAb, Mouse

THE™ beta Actin Antibody [HRP], mAb, Mouse

Synonyms THETM beta Actin Antibody [HRP], mAb, Mouse;
Description GenScript THETM beta Actin Antibody [HRP], mAb, Mouse is HRP conjugated THETM beta Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein G affinity column. This product provides a useful and serves as a specific tool in the study of the intracellular distribution of ß-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.
Host Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein G affinity column

Subclass Mouse IgG2a
Clone ID 2D1D10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM beta Actin Antibody [HRP], mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

  • THE™ Beta Actin Antibody [HRP], MAb, Mouse
  • THE™ Beta Actin Antibody [HRP], MAb, Mouse

    Detection antibody: 1 μg/ml THETM Anti-β-Actin [HRP] Monoclonal Antibody (Mouse) (GenScript, A00730)

    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500).

Cat. No. Product Name Quantity Price
α-Actin-1 Antibody, pAb, Rabbit - 40 μg
THE™ beta Actin Antibody [HRP], mAb, Mouse - 200 μg
THE™ beta Actin Antibody [HRP], mAb, Mouse - 100 μg
THE™ beta Actin Antibody, mAb, Mouse - 100 μg
THE™ beta Actin Antibody, mAb, Mouse - 200 μg
Lee I, et al. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proc Natl Acad Sci U S A. 2014Jan.;

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Berry WL.,et al. Oncogenic features of the JMJD2A histone demethylase in breast cancer.Int J Oncol.2012Nov;41(5):1701-6

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Luisa F. Jiménez-Soto.,et al. Dynamics of the Cag‐Type IV Secretion System of Helicobacter pylori as studied by bacterial co‐infections.Cell Microbiol.2013Jul;

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Cat. No. A00730
Size Price
100 μg $180.00
200 μg $333.00