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THETM c-Myc Antibody [HRP], mAb, Mouse

Synonyms Mouse Anti-c-Myc [HRP] mAb;
Description GenScript THETM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass Mouse IgG2a
Clone ID 2G8D5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

 THE<sup>TM</sup> C-Myc Antibody [HRP], MAb, Mouse
 THE<sup>TM</sup> C-Myc Antibody [HRP], MAb, Mouse

Lane 1 and 2: Multiple Tag Cell Lysate (GenScript, M0100)
Primary antibody: 0.1 μg/ml THETM Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) (GenScript, A00863)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

Cat. No.
Product Name
A00172-40 c-Myc-tag Antibody, pAb, Rabbit
40 ug
A00173-100 c-Myc-tag Antibody [HRP], pAb, Rabbit
100 ug
A00173-40 c-Myc-tag Antibody [HRP], pAb, Rabbit
40 ug
A00704-100 THETM c-Myc Tag Antibody, mAb, Mouse
100 ug
A00864-40 THETM c-Myc Antibody [Biotin], mAb, Mouse
40 ug
A00864-200 THETM c-Myc Antibody [Biotin], mAb, Mouse
200 ug

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Cat. No. A00863
100 ug $180.00
24 hour