Catalog Products » Antibodies » THE™ Elite Antibodies » THE^TM c-Myc Antibody [Biotin], mAb, Mouse

THE^TM c-Myc Antibody [Biotin], mAb, Mouse

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Overview

Synonyms	Mouse Anti-c-Myc [Biotin] mAb;
Description	GenScript THE^TM c-Myc Antibody [Biotin], mAb, Mouse is the biotin conjugated THE^TM Anti-c-Myc Monoclonal Antibody (Mouse) (A00704) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Host Species	Mouse
Conjugation	Biotin
Immunogen	A synthetic peptide EQKLISEEDL coupled to KLH
Fusion Partner	Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Purification	Protein A affinity column

Properties

Subclass	IgG2a, k
Clone ID	2G8D5
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity	GenScript THE^TM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> C-Myc Antibody [Biotin], MAb, Mouse

Lane 1-3: 200 ng, 50 ng, 10 ng Multiple Tag Cell Lysate (GenScript, M0100)
Lane 4: Negative Control
Detect antibody: 0.1 µg/ml THE^TM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (GenScript, A00864)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Related Products

Cat. No.	Product Name	Price
A00172-40	c-Myc-tag Antibody, pAb, Rabbit - 40 ug	$99.00
A00173-40	c-Myc-tag Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
A00864-40	THE^TM c-Myc Antibody [Biotin], mAb, Mouse - 40 ug	$74.00
A00864-200	THE^TM c-Myc Antibody [Biotin], mAb, Mouse - 200 ug	$333.00
A00863-100	THE^TM c-Myc Antibody [HRP], mAb, Mouse - 100 ug	$180.00

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Armstrong GA.,et al. Glial Hsp70 Protects K+ Homeostasis In The Drosophila Brain During Repetitive Anoxic Depolarization.PLoS One.2011Dec;6(12):e28994

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