×
Catalog Products » Antibodies » THE™ Elite Antibodies » THETM c-Myc Antibody [Biotin], mAb, Mouse

THETM c-Myc Antibody [Biotin], mAb, Mouse

Synonyms Mouse Anti-c-Myc [Biotin] mAb;
Description GenScript THETM c-Myc Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM Anti-c-Myc Monoclonal Antibody (Mouse) (A00704) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Host Species Mouse
Conjugation Biotin
Immunogen A synthetic peptide EQKLISEEDL coupled to KLH
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG2a, k
Clone ID 2G8D5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript THETM c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> C-Myc Antibody [Biotin], MAb, Mouse
 THE<sup>TM</sup> C-Myc Antibody [Biotin], MAb, Mouse

Lane 1-3: 200 ng, 50 ng, 10 ng Multiple Tag Cell Lysate (GenScript, M0100)
Lane 4: Negative Control
Detect antibody: 0.1 µg/ml THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (GenScript, A00864)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Cat. No.
Product Name
Size
Price
Quantity
A00172-40 c-Myc-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00173-40 c-Myc-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A00704-100 THETM c-Myc Tag Antibody, mAb, Mouse
100 ug
$175.00
A00864-40 THETM c-Myc Antibody [Biotin], mAb, Mouse
40 ug
$74.00
A00864-200 THETM c-Myc Antibody [Biotin], mAb, Mouse
200 ug
$333.00
A00863-100 THETM c-Myc Antibody [HRP], mAb, Mouse
100 ug
$180.00
 
Armstrong GA.,et al. Glial Hsp70 Protects K+ Homeostasis In The Drosophila Brain During Repetitive Anoxic Depolarization.PLoS One.2011Dec;6(12):e28994

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A00864
Size
Price
40 ug $74.00
200 ug $333.00
24 hour
 
*
*
*
*