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THE™ GST Antibody, mAb, Mouse

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Western blot analysis of GST-tagged fusion proteins using THE^TM GST Antibody, mAb, Mouse (GenScript, A00865, 1 µg/ml)
Lane 1: N-terminal GST tagged fusion protein
Lane 2: Internal GST tagged fusion protein
Lane 3: C-terminal GST tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221).

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Overview

Specificity	THE™ Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Host Species	Mouse
Immunogen	Recombinant GST protein (26 kD)
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Immunoprecipitation (IP)	1 µg/ml
Other applications	User-optimized

Properties

Form	Lyophilized
Storage Buffer	1.40 µg, 100 µg, 200 µg (GenScript, A00865), Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.2.500 µg (GenScript, A00865), Lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	2F10B9

Examples

Background

Target Background

Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles.GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E.coli.The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione.Elution can be accomplished using free glutathione.The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases.Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

Synonyms

Mouse Anti-GST monoclonal antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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