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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM GST Antibody, mAb, Mouse

THE™ GST Antibody, mAb, Mouse

THE™ GST Antibody, MAb, Mouse

Western blot analysis of GST-tagged fusion proteins using THETM GST Antibody, mAb, Mouse (GenScript, A00865, 1 µg/ml)
Lane 1: N-terminal GST tagged fusion protein
Lane 2: Internal GST tagged fusion protein
Lane 3: C-terminal GST tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant GST protein (26 kD)
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column
Specificity THETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.

Subclass IgG1, k
Clone ID 2F10B9
Concentration 1. 40 µg, 100 µg, 200 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
2. 500 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2 ml of PBST).
Immunoprecipitation (IP): 1 µg/ml
Other applications: user-optimized

  • THE™ GST Antibody, MAb, Mouse
  • THE™ GST Antibody, MAb, Mouse

    Western blot analysis of GST-tagged fusion proteins using THETM GST Antibody, mAb, Mouse (GenScript, A00865, 1 µg/ml)
    Lane 1: N-terminal GST tagged fusion protein
    Lane 2: Internal GST tagged fusion protein
    Lane 3: C-terminal GST tagged fusion protein
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).


Synonyms Mouse Anti-GST monoclonal antibody;
Target Background Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

Cat. No. Product Name Quantity Price
Z02039-1
Glutathione S-Transferase - 1 mg
$65.00
L00411
GST tag ELISA Detection Kit - 1 kit (1x96 well)
$298.00
L00207
GST Fusion Protein Purification Kit - 1 kit
$139.00
A00867-200
THE™ GST Antibody [Biotin], mAb, Mouse - 200 μg
$333.00
A00866-100
THE™ GST Antibody [HRP], mAb, Mouse - 100 μg
$180.00
A00865-100
THE™ GST Antibody, mAb, Mouse - 100 μg
$175.00
A00865-200
THE™ GST Antibody, mAb, Mouse - 200 μg
$311.00
A00865-500
THE™ GST Antibody, mAb, Mouse - 500 μg
$750.00
A00130-40
GST-tag Antibody [HRP], pAb, Rabbit - 40 μg
$99.00
 
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Muzammel Haque and Konstantin G. Kousoulas., et al. The Kaposi's Sarcoma-Associated Herpesvirus ORF34 Protein Binds to HIF-1α and Causes Its Degradation via the Proteasome Pathway. J Virol. (2013-02)

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Cat. No. A00865
Size Price
100 μg $175.00
200 μg $311.00
500 μg $750.00