×
Catalog Products » Antibodies » THE™ Elite Antibodies » THETM GST Antibody [HRP], mAb, Mouse

THETM GST Antibody [HRP], mAb, Mouse

Synonyms Mouse Anti-GST [HRP] mAb;
Description GenScript THETM GST Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of GST in fusion proteins.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen Recombinant GST protein (26 kD)
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1
Clone ID 2F10B9
Concentration 0.5mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity THETM GST Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> GST Antibody [HRP], MAb, Mouse
 THE<sup>TM</sup> GST Antibody [HRP], MAb, Mouse

Western blot analysis of GST-tagged fusion protein using THETM GST Antibody [HRP], mAb, Mouse (GenScript, A00866, 0.1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 26 kD
Observed Size: 26 kD


Cat. No.
Product Name
Size
Price
Quantity
A00866-40 THETM GST Antibody [HRP], mAb, Mouse
40 ug
$74.00
A01380-40 GST-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
L00207 GST Fusion Protein Purification Kit
1 kit
$139.00
L00411 GST tag ELISA Detection Kit
1 kit (1x96 well)
$298.00
A00866-100 THETM GST Antibody [HRP], mAb, Mouse
100 ug
$180.00
A00097-100 GST-tag Antibody, pAb, Rabbit
100 ug
$199.00
A01379-100 GST-tag Antibody, pAb, Goat
100 ug
$199.00
A01380-100 GST-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
 
Zhang Y.,et al. A quartet of PIF bHLH factors provides a transcriptionally centered signaling hub that regulates seedling morphogenesis through differential expression-patterning of shared target genes in Arabidopsis.PLoS Genet.2013Jan;

Park S.,et al. The CH2 domain of CBP/p300 is a novel zinc finger.FEBS Lett.2013Jul;

Barbelanne M.,et al. Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis.Hum Mol Genet.2013Jun;22(12):2482 - 2494

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A00866
Size
Price
40 ug $74.00
100 ug $180.00
24 hour
 
*
*
*
*