Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ GST Antibody [Biotin], mAb, Mouse
THE™ GST Antibody [Biotin], MAb, Mouse

Western blot analysis of GST-tagged fusion protein using THETM GST Antibody [Biotin], mAb, Mouse (GenScript, A00867, 1 µg/ml)
The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 31 kD
Observed Size: 31 kD

THE™ GST Antibody [Biotin], mAb, Mouse

THE™ GST Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
A00867
$333.00

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Overview
Specificity THE™ GST Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Host Species Mouse
Immunogen Recombinant GST protein (26 kD)
Conjugate Biotin

Applications
Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 2F10B9

Examples
  • THE™ GST Antibody [Biotin], MAb, Mouse
    • THE™ GST Antibody [Biotin], MAb, Mouse

    Western blot analysis of GST-tagged fusion protein using THETM GST Antibody [Biotin], mAb, Mouse (GenScript, A00867, 1 µg/ml)
    The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
    Predicted Size: 31 kD
    Observed Size: 31 kD


Background
Target Background GenScript THE™ GST Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column.The antibody is suitable for detecting the expression level of GST in fusion proteins.
Synonyms Mouse Anti-GST-tag [Biotin] mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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