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THETM GST Antibody [Biotin], mAb, Mouse

Synonyms Mouse Anti-GST-tag [Biotin] mAb;
Description GenScript THETM GST Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM Anti-GST Monoclonal Antibody (Mouse) (A00865) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of GST in fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen Recombinant GST protein (26 kD)
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 2F10B9
Concentration Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity THETM GST Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  •  THE<sup>TM</sup> GST Antibody [Biotin], MAb, Mouse
  •  THE<sup>TM</sup> GST Antibody [Biotin], MAb, Mouse

    Western blot analysis of GST-tagged fusion protein using THETM GST Antibody [Biotin], mAb, Mouse (GenScript, A00867, 1 µg/ml)
    The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
    Predicted Size: 31 kD
    Observed Size: 31 kD

Cat. No.
Product Name
GST-tag Antibody [HRP], pAb, Rabbit - 40 ug
GST-tag Antibody [Biotin], pAb, Rabbit - 100 ug
THETM GST Antibody, mAb, Mouse - 40 ug
THETM GST Antibody, mAb, Mouse - 200 ug
THETM GST Antibody, mAb, Mouse - 500 ug
THETM GST Antibody [HRP], mAb, Mouse - 40 ug
GST-tag Antibody [HRP], pAb, Goat - 40 ug
GST-tag Antibody, pAb, Rabbit - 100 ug
GST-tag Antibody [HRP], pAb, Goat - 100 ug

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Cat. No. A00867
40 ug $74.00
200 ug $333.00