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Catalog Products » Antibodies » Epitope Tag Antibodies » NWSHPQFEK Antibody [HRP], pAb, Rabbit

NWSHPQFEK Antibody [HRP], pAb, Rabbit

Synonyms Rabbit anti NWSHPQFEK [HRP] pAb;
Description Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.
Host Species Rabbit
Conjugation Horseradish Peroxidase (HRP)
Immunogen Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.
Purification Immunoaffinity

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity This Antibody recognizes Strep-tag II tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other Applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

NWSHPQFEK Antibody [HRP], PAb, Rabbit
NWSHPQFEK Antibody [HRP], PAb, Rabbit

Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody [HRP], pAb, Rabbit (GenScript, A00875, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 60 kD
Observed Size: 60 kD


Cat. No.
Product Name
Size
Price
Quantity
A00875-40 NWSHPQFEK Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A01732-100 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
100 ug
$319.00
A01732-200 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
200 ug
$579.00
A01736-100 THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse
100 ug
$349.00
A01737-100 THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse
100 ug
$349.00
A01742-40 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
40 ug
$154.00
A01742-100 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
100 ug
$349.00
A01742-200 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
200 ug
$637.00
 


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Cat. No. A00875
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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