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Catalog Products » Antibodies » Epitope Tag Antibodies » V5-tag Antibody [HRP], pAb, Rabbit

V5-tag Antibody [HRP], pAb, Rabbit

Synonyms Rabbit Anti-V5-tag [HRP] pAb;
Description GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
Host Species Rabbit
Conjugation Horseradish Peroxidase (HRP)
Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 40μg:1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
100 μg:0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

V5-tag Antibody [HRP], PAb, Rabbit
V5-tag Antibody [HRP], PAb, Rabbit

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody [HRP], pAb, Rabbit (GenScript, A00877, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD


Cat. No.
Product Name
Size
Price
Quantity
A00623-100 V5-tag Antibody, pAb, Rabbit
100 ug
$199.00
A00877-40 V5-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A01724-40 THETM V5 Tag Antibody, mAb, Mouse
40 ug
$119.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 ug
$219.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 ug
$395.00
A01733-100 THETM V5 Tag Antibody [HRP], mAb, Mouse
100 ug
$239.00
A01735-100 THETM V5 Tag Antibody [Biotin], mAb, Mouse
100 ug
$239.00
A01803-100 THETM V5 Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$263.00
A01804-100 THETM V5 Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$263.00
A01805-100 THETM V5 Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$263.00
 
Tahir SA.,et al. Caveolin-1-LRP6 Signaling Module Stimulates Aerobic Glycolysis in Prostate Cancer.Cancer Res.2013Jan;

Zhang K.,et al. The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit.J Cell Biol.2013Mar;200(6):807 - 820

Dahl LD, et al. An EF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus. Cancer Cell Int. 2014Feb.;14(1):17

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Cat. No. A00877
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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