Presenilin 1 Antibody, pAb, Rabbit

*This product has been discontinued!*

GenScript Rabbit Anti-Presenilin 1 Polyclonal Antibody can detect endogenous levels of the full-length protein (approx.43-52 kD) and the N-terminal fragment of presenilin 1 (≈ 28 kD).

Cat. No.	A00881
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript Rabbit Anti-Presenilin 1 Polyclonal Antibody can detect endogenous levels of the full-length protein (approx.43-52 kD) and the N-terminal fragment of presenilin 1 (≈ 28 kD).
Host Species	Rabbit
Immunogen	Synthetic peptide corresponding to amino acids 25-50 (TVRSQNDNRERQEHNDRRSLGHPEPL) of the N-terminus of human presenilin 1, which is exposed to the extracellular region, KLH conjugated.
Species Reactivity	Human, mouse
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.5-2.0 µg/ml

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Properties

Form	Liquid
Storage Buffer	1×PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Concentration	0.5 mg/ml
Storage Instructions	The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable
Note	Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.

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Background

Target Background

Presenilin 1 (PS1) and presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein).The other members of the gamma-secretase complex are required to have protease activity.PS1 and PS2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane.It regulates epithelial-cadherin function.Three causative genes have been identified that when mutated lead to presenile Alzheimer's disease: APP (amyloid precursor protein gene), PSEN1, and PSEN2.The presenilin 1 gene has recently been discovered on chromosome 14 and is responsible for an early onset form of Alzheimer's disease that accounts for 70-80% of the inherited forms of Alzheimer's.It is a membrane protein that has a region where at least seven mutations have been identified in family members with Alzheimer's disease.Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (28 kD) and carboxy-terminal (≈17 kD) fragments.GenScript Rabbit Anti-Presenilin 1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, TVRSQNDNRERQEHNDRRSLGHPEPL, corresponding to amino acids 25-50 of the N-terminus of human presenilin 1.Rabbit Anti-Presenilin 1 Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.

Synonyms

Rabbit anti-PS1 pAb;

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Puri RV, et al. Notch4 activation aggravates NF-κB-mediated inflammation in HIV-1-associated nephropathy. Disease models & mechanisms. (2019-12)