|Synonyms||Rabbit Anti-C-X-C chemokine receptor type 4 pAb;|
|Description||CXCR4 is one member of G-protein coupled receptor that contains seven transmembrane spanning. CXCR4 is a principal coreceptor for T cell tropic strains of HIV1 fusion and entry of human white blood cells. It is also required for the infection by dual-tropic strains of HIV1 and mediates CD4 independent infection by HIV2. Chemokine SDF1 is the ligand for CXCR4 that prevents infection by T tropic HIV. CXCR4 is highly expressed in brain, heart, white blood cells, vascular endothelial cells, and umbilical cord endothelial cells.
GenScript Rabbit Anti-CXCR4 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human CXCR4 (Swiss Prot: P61073).
|Immunogen||KLH-coupled synthetic peptide from N-terminal of human CXCR4 (Swiss Prot: P61073)|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-CXCR4 Polyclonal Antibody detects endogenous levels of human and mouse CXCR4.
Positive Control: Hela, HEK293.
|Species Reactivity||Human and mouse. This product has not been tested for other species yet.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized
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