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Catalog Products » Antibodies » Epitope Tag Antibodies » Ty1-tag Antibody, pAb, Rabbit

Ty1-tag Antibody, pAb, Rabbit

Ty1-tag Antibody, PAb, Rabbit

Western blot analysis of Ty1 tagged fusion protein using Ty1-tag Antibody, pAb, Rabbit (GenScript, A01004, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 60 kD
Observed Size: 60 kD

Ty1-tag Antibody, PAb, Rabbit

Western blot analysis of Ty1 tagged fusion proteins expressed in E. coli cell lysate using Ty1-tag Antibody (GenScript, A01004, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-Ty1-tag pAb;
Description Well-characterized antibodies for epitope tags are common in the study of protein expression in various systems.
GenScript Rabbit Anti- Ty1-tag Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Ty1-tag epitope peptide conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity This Antibody recognizes both Ty1 tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-2 µg/ml
Immunoprecipitation (IP): 2-5 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

Ty1-tag Antibody, PAb, Rabbit
Ty1-tag Antibody, PAb, Rabbit

Western blot analysis of Ty1 tagged fusion protein using Ty1-tag Antibody, pAb, Rabbit (GenScript, A01004, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 60 kD
Observed Size: 60 kD

Ty1-tag Antibody, PAb, Rabbit
Ty1-tag Antibody, PAb, Rabbit

Western blot analysis of Ty1 tagged fusion proteins expressed in E. coli cell lysate using Ty1-tag Antibody (GenScript, A01004, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A01004
Size
Price
40 ug $99.00
 
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