THE™ HA Tag Antibody, mAb, Mouse
Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate
Primary antibody:
A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)
Secondary antibody:
IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG
293 cells transfected with N-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)
293 cells transfected with C-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)
Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
| Specificity | THE™ HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins. |
| Host Species | Mouse |
| Immunogen | A KLH coupled synthetic peptide (YPYDVPDYA) |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||||||||
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1 |
| Clonality | Monoclonal |
| Clone ID | 5E11D8 |
| Note | GenScript can offer this product per customer's reuqest including product size, buffer components,etc. |
Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate
Primary antibody:
A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)
Secondary antibody:
IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG
293 cells transfected with N-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)
293 cells transfected with C-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)
Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
| Target Background | GenScript THE™ HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THE™ elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. |
| Synonyms | Mouse Anti-HA-Tag Monoclonal Antibody; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01808-100 |
THE™ HA Tag Antibody [iFluor 647], mAb, Mouse - 100 μg |
$344.00 |
|
A01806-100 |
THE™ HA Tag Antibody [iFluor 488], mAb, Mouse - 100 μg |
$344.00 |
|
A01621 |
THE™ HA Tag Antibody [FITC], mAb, Mouse - 100 μg |
$299.00 |
|
A01296 |
THE™ HA Tag Antibody [HRP], mAb, Mouse - 100 μg |
$299.00 |
Sheng-Li Ming , et al. Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy. Autophagy. (2021-11)
Tong Zhu, et al. Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana. Mol Plant Pathol. (2021-10)
Weixiang Bian, et al. Low-density-lipoprotein-receptor-related protein 1 mediates Notch pathway activation. Dev Cell. (2021-10)
Soumitra S Datta, et al. Psychological interventions for psychosis in adolescents. Cochrane Database Syst Rev. (2020-07-01)
NakagawaNaoki, et al. Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development. Neuron. (2019-06)
Hsu Shih-Chi, et al. N-terminal sequences affect expression of triterpene biosynthesis enzymes in Chlamydomonas chloroplasts. Algal Research. (2019)
Ghosal D, et al. Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nat Microbiol. (2019)
Camacho-VillegasTanya A, et al. Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF Neutralization. Mar Drugs. (2018)
Cho Hyunju, et al. Substrate relay in an Hsp70-cochaperone cascade safeguards tail-anchored membrane protein targeting. EMBO J. (2018)
Bui M, et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics & chromatin. (2017-01)
Arthur Laura L, et al. Rapid generation of hypomorphic mutations. Nat Commun. (2017)
Cai D, et al. Establishment of an inducible HBV stable cell line that expresses cccDNA-dependent epitope-tagged HBeAg for screening of cccDNA modulators. Antiviral Res. (2016)
He Y, et al. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. (2016)
Y Liu, et al. Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus-producing hepatoma cells. Mol Med Rep. (2014-12)
Swaney DL, et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. (2013-06)
Yulia V. Shulga, et al. Phosphatidylinositol-4-Phosphate 5-Kinase Isoforms Exhibit Acyl Chain Selectivity For Both Substrate And Lipid Activator. J Biol Chem. (2012-10)
Dasiel O. Borroto-Escuela, et al. Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation. Cell Physiol Biochem. (2011-12)
You can visit "Citations Database" for more citations.
Tong Zhu, et al. Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana. Mol Plant Pathol. (2021-10)
Weixiang Bian, et al. Low-density-lipoprotein-receptor-related protein 1 mediates Notch pathway activation. Dev Cell. (2021-10)
Soumitra S Datta, et al. Psychological interventions for psychosis in adolescents. Cochrane Database Syst Rev. (2020-07-01)
NakagawaNaoki, et al. Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development. Neuron. (2019-06)
Hsu Shih-Chi, et al. N-terminal sequences affect expression of triterpene biosynthesis enzymes in Chlamydomonas chloroplasts. Algal Research. (2019)
Ghosal D, et al. Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nat Microbiol. (2019)
Camacho-VillegasTanya A, et al. Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF Neutralization. Mar Drugs. (2018)
Cho Hyunju, et al. Substrate relay in an Hsp70-cochaperone cascade safeguards tail-anchored membrane protein targeting. EMBO J. (2018)
Bui M, et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics & chromatin. (2017-01)
Arthur Laura L, et al. Rapid generation of hypomorphic mutations. Nat Commun. (2017)
Cai D, et al. Establishment of an inducible HBV stable cell line that expresses cccDNA-dependent epitope-tagged HBeAg for screening of cccDNA modulators. Antiviral Res. (2016)
He Y, et al. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. (2016)
Y Liu, et al. Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus-producing hepatoma cells. Mol Med Rep. (2014-12)
Swaney DL, et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. (2013-06)
Yulia V. Shulga, et al. Phosphatidylinositol-4-Phosphate 5-Kinase Isoforms Exhibit Acyl Chain Selectivity For Both Substrate And Lipid Activator. J Biol Chem. (2012-10)
Dasiel O. Borroto-Escuela, et al. Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation. Cell Physiol Biochem. (2011-12)
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A01244 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $287.00 | |
