TF-tag Antibody, pAb, Rabbit
Western blot analysis of TF-tagged fusion protein using TF-tag Antibody, pAb, Rabbit (GenScript, A01329, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 59 kD
Observed Size: 59 kD
Lane 1 (Rabbit IgG): Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 2 (E.coil lysate): Immunprecipitation with TF-tag Antibody, pAb, Rabbit (GenScript, A01329)
Lane 3 (E.coil lysate): Input control material for lysate expressing TF-tagged fusion protein
| Synonyms | Rabbit Anti-TF-tag pAb; |
| Description | Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins. GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant TF protein |
| Purification | Immunoaffinity chromatography |
| Subclass | Rabbit IgG |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
| Specificity | GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.2-1 µg/ml Immunoprecipitation (IP): 2-10 µg/mg of lysate Other Applications: user-optimized |
Western blot analysis of TF-tagged fusion protein using TF-tag Antibody, pAb, Rabbit (GenScript, A01329, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 59 kD
Observed Size: 59 kD
Lane 1 (Rabbit IgG): Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 2 (E.coil lysate): Immunprecipitation with TF-tag Antibody, pAb, Rabbit (GenScript, A01329)
Lane 3 (E.coil lysate): Input control material for lysate expressing TF-tagged fusion protein
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01388-40 |
GFP Antibody, pAb, Rabbit - 40 ug |
$99.00 |
Lin KF., et al. Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment. Biophys J. 2012 Jun;102(12):2818-27.
Eunyong Park and Tom A. Rapoport. Bacterial Protein Translocation Requires Only One Copy Of The Secy Complex In Vivo. J Cell Biol. 2012 Sep;198(5):881 - 893.
You can visit "Citations Database" for more citations.
Eunyong Park and Tom A. Rapoport. Bacterial Protein Translocation Requires Only One Copy Of The Secy Complex In Vivo. J Cell Biol. 2012 Sep;198(5):881 - 893.
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A01329 | ||
|---|---|---|
| Size | Price | |
| 40 ug | $99.00 | |
| 100 ug | $199.00 | |
