Western blot analysis of TF-tagged fusion protein using TF-tag Antibody, pAb, Rabbit (GenScript, A01329, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 59 kD
Observed Size: 59 kD
|Synonyms||Rabbit Anti-TF-tag pAb;|
|Description||Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins.
GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
|Immunogen||Purified recombinant TF protein|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
IF & ICC Protocol(pdf)