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Catalog Products » Antibodies » Epitope Tag Antibodies » TF-tag Antibody, pAb, Rabbit

TF-tag Antibody, pAb, Rabbit

TF-tag Antibody, PAb, Rabbit

Western blot analysis of TF-tagged fusion protein using TF-tag Antibody, pAb, Rabbit (GenScript, A01329, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 59 kD
Observed Size: 59 kD

TF-tag Antibody, PAb, Rabbit

Synonyms Rabbit Anti-TF-tag pAb;
Description Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins.

GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Purified recombinant TF protein
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

TF-tag Antibody, PAb, Rabbit
TF-tag Antibody, PAb, Rabbit

Western blot analysis of TF-tagged fusion protein using TF-tag Antibody, pAb, Rabbit (GenScript, A01329, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 59 kD
Observed Size: 59 kD

TF-tag Antibody, PAb, Rabbit
TF-tag Antibody, PAb, Rabbit


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Eunyong Park and Tom A. Rapoport. Bacterial Protein Translocation Requires Only One Copy Of The Secy Complex In Vivo. J Cell Biol. 2012 Sep;198(5):881 - 893.

Lin KF., et al. Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment. Biophys J. 2012 Jun;102(12):2818-27.

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For more documents, please visit "Technical Support".

Cat. No. A01329
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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