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Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody [HRP], pAb, Goat

GST-tag Antibody [HRP], pAb, Goat

Synonyms Goat Anti-GST-tag [HRP] pAb;
Description Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.

GenScript Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.
Host Species Goat
Conjugation Horseradish Peroxidase (HRP)
Immunogen Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
Purification Immunoaffinity chromatography

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml
Specificity GenScript Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Other Applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)

GST-tag Antibody [HRP], PAb, Goat
GST-tag Antibody [HRP], PAb, Goat

Western blot analysis of GST-tagged fusion protein using GST-tag Antibody [HRP], pAb, Goat (GenScript, A01380, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V)
Predicted Size: 31 kD
Observed Size: 31 kD, 62kD


Cat. No.
Product Name
Size
Price
Quantity
A00130-100 GST-tag Antibody [HRP], pAb, Rabbit
100 ug
$199.00
A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
100 ug
$199.00
A00202-40 GST-tag Antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 ug
$175.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 ug
$311.00
A00865-500 THETM GST Antibody, mAb, Mouse
500 ug
$750.00
A00867-40 THETM GST Antibody [Biotin], mAb, Mouse
40 ug
$74.00
A00867-200 THETM GST Antibody [Biotin], mAb, Mouse
200 ug
$333.00
L00411 GST tag ELISA Detection Kit
1 kit (1x96 well)
$298.00
A00866-100 THETM GST Antibody [HRP], mAb, Mouse
100 ug
$180.00
A00097-100 GST-tag Antibody, pAb, Rabbit
100 ug
$199.00
A01379-100 GST-tag Antibody, pAb, Goat
100 ug
$199.00
A01380-100 GST-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
 
Gish LA., et al. WUSCHEL-Responsive At5g65480 Interacts with CLAVATA Components In Vitro and in Transient Expression. PLoS One. 2013 Jun;8(6):e66345.

NS Savar, et al. In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary tract infections. J B

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Cat. No. A01380
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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