Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

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Loading: N-terminal DYKDDDDK-fusion protein (400 ng, 100 ng, 25 ng)
Primary antibody: 1 μg/ml THE^TM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Biotin) (GenScript, Cat. No. A01429, Lot No. A213902)
Secondary antibody: Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091)

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Overview

Specificity	THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
Host Species	Mouse
Immunogen	A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate	Biotin

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Other applications	User-optimized

Properties

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2b,κ
Clonality	Monoclonal
Clone ID	5A8E5

Examples

Background

Target Background	GenScript THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.
Synonyms	anti Flag-tag mAb; anti DYKDDDDK-tag mAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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