×
Catalog Products » Antibodies » THE™ Elite Antibodies » THETM alpha Tubulin Antibody [HRP], mAb, Mouse

THETM alpha Tubulin Antibody [HRP], mAb, Mouse

Synonyms THETM Anti-α-tubulin [HRP] mAb (mouse);
Description THETM α Tubulin Antibody [HRP], mAb, Mouse is HRP conjugated to THETM α Tubulin Antibody [HRP], mAb, Mouse (Cat. No. A01410) purified from mouse ascites by protein A affinity column. The product is a specific and useful tool in studying proper interpretation of western blots.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen KLH-coupled synthetic peptide within residues 400-450 of human α-tubulin (Swiss Prot: P68363).
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Subclass Mouse IgG2b, κ
Clone ID 1F4E10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM alpha Tubulin Antibody [HRP], mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.5 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> Alpha Tubulin Antibody [HRP], MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody [HRP], MAb, Mouse

Western blot analysis of cell lysates using THETM alpha Tubulin Antibody [HRP], mAb, Mouse (GenScript, A01490, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 50 kD
Observed Size: 50 kD


Cat. No.
Product Name
Size
Price
Quantity
A01203-40 β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01410-100 THETM alpha Tubulin Antibody, mAb, Mouse
100 ug
$225.00
A01410-40 THETM alpha Tubulin Antibody, mAb, Mouse
40 ug
$108.00
A01545 THETM alpha Tubulin Antibody [Biotin], mAb, Mouse
100 ug
$335.00
A01627-40 Neuron specific β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01717-40 β-tubulin Antibody (2G7D4), mAb, Mouse
40 ug
$99.00
 
Gupta R., et al. Squelching of ETS2 transactivation by POU5F1 silences the human chorionic gonadotropin CGA subunit gene in human choriocarcinoma and embryonic stem cells. Mol Endocrinol. 2012 May;26(5):859-72.

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A01490
Size
Price
100 ug $335.00
24 hour
 
*
*
*
*