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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM cGMP Antibody, mAb, Mouse

THETM cGMP Antibody, mAb, Mouse

Synonyms THETM cGMP Antibody, mAb, Mouse;
Description Guanosine 3, 5-cyclic monophosphate (cyclic GMP; cGMP) acts as a secondary messenger much like cyclic AMP. It is generally known to activate intracellular protein kinases in response to the binding of membrane-impermeable peptide hormones to the cell surface. cGMP synthesis is catalyzed by guanylate cyclase (GC), which converts GTP to cGMP. Membrane-bound GC is activated by peptide hormones such as atrial natriuretic factor, while soluble GC is typically activated by nitric oxide (NO) to stimulate cGMP synthesis. cGMP is also a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. It also relaxes smooth muscle tissues. The roles of cGMP and cAMP may be linked, as evidenced by the fact that some cellular functions are controlled bi-directionally by both cAMP and cGMP. Some functions are stimulated by cGMP and suppressed by cAMP and vice versa.
GenScript THETM cGMP Antibody, mAb, Mouse is developed using 3, 5-cyclic GMP-8-KLH as immunogen.
Host Species Mouse
Conjugation Unconjugated
Immunogen 3', 5'-cyclic GMP-8-KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass Mouse IgG2a, κ
Clone ID 2F11E10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.04-0.1 µg/ml
Other: user-optimized
This antibody is derived from immunization of cyclic GMP derivative conjugated to KLH at position 8 of the guanine structure. It may not react well with cGMP conjugated via other positions.
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> CGMP Antibody, MAb, Mouse
THE<sup>TM</sup> CGMP Antibody, MAb, Mouse

cGMP Competitive ELISA
1.ELISA plate was coated with goat anti mouse IgG antibody
2.Anti-cGMP monoclonal antibody (Cat. No. A01508) at appropriate dilution and cGMP standards or testing compounds were added into appropriate reaction wells.
3.After a period of incubation, cGMP-HRP conjugate (Cat.No.M01058) was added followed by proper period of incubation.
4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.


Cat. No.
Product Name
Size
Price
Quantity
A00615-200 cGMP Antibody, pAb, Rabbit
200 μl
$349.00
A00615-40 cGMP Antibody, pAb, Rabbit
40 μl
$99.00
M01058 cGMP-HRP
200 μl
$105.00
 


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Cat. No. A01508
Size
Price
100 ug $287.00
24 hour
 
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