EEA1 antibody, pAb, Rabbit

*This product has been discontinued!*

GenScript Rabbit Anti-EEA1 Polyclonal Antibody detects endogenous levels of human, mouse and rat EEA1 protein.

Cat. No.	A01514
Size	Buy in bulk

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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript Rabbit Anti-EEA1 Polyclonal Antibody detects endogenous levels of human, mouse and rat EEA1 protein.
Host Species	Rabbit
Immunogen	KLH-coupled synthetic peptide from N-terminal of human EEA1 (Swiss Prot: Q15075).
Species Reactivity	Human, mouse and rat
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Immunohistochemistry	5-10 µg/ml
Western Blot	1-2 µg/ml
Other applications	User-optimized

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Properties

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable

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Background

Target Background

Early endosomal antigen 1 (EEA1) is an early endosomal marker which is essential for fusion between early endocytic vesicles. It interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion. EEA1 protein contains an N-terminal zinc finger motif and a cys-rich C-terminal metal-binding finger. A FYVE domain located at the carboxy terminus of EEA1 binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes. GenScript Rabbit Anti-EEA1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human EEA1 (Swiss Prot: Q15075).

Synonyms

Rabbit Anti-EEA1 polyclonal antibody ; Early endosome antigen 1 antibody

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Lidiya P Dubytska, et al. Early Intracellular Trafficking and Subsequent Activity of Programmed Cell Death in Channel Catfish Macrophages Infected with. Microorganisms. (2020)