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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM alpha Tubulin Antibody [Biotin], mAb, Mouse

THETM alpha Tubulin Antibody [Biotin], mAb, Mouse

THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse

Loading:
1. 40µg pig muscle cell lysate
2. 40µg CHO cell lysate
3. 40µg COS-7 cell lysate
4. 40µg rabbit kidney cell lysate
5. 40µg Hela cell lysate
Primary antibody: 0.5 µg/ml THETM α-tubulin Antibody[Biotin], mAb, Mouse (GenScript, A01545)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)

THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse

Loading:
1. 60µg Hela cell lysate
2. 12µg Hela cell lysate
3. 2.4µg Hela cell lysate
Primary antibody: 0.5 µg/ml THETM α-tubulin Antibody[Biotin], mAb, Mouse (GenScript, A01545)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)

Synonyms THE™ α-Tubulin Antibody [Biotin], mAb, Mouse;
Description GenScript THETM α-tubulin Antibody[Biotin], mAb, Mouse is the biotin conjugated THETM α-tubulin Antibody, mAb, Mouse(A01410) purified from mice ascites by protein A affinity column.
Host Species Mouse
Conjugation Biotin
Immunogen KLH-coupled synthetic peptide within residues 400-450 of human α-tubulin (Swiss Prot: P68363).
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Subclass Mouse IgG2b, k
Clone ID 1F4E3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript THETM α-tubulin Antibody[Biotin], mAb, Mouse recognizes Mouse, Human, Hamster, Monkey, Rabbit and Pig. The antibody has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.5 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse

Loading:
1. 40µg pig muscle cell lysate
2. 40µg CHO cell lysate
3. 40µg COS-7 cell lysate
4. 40µg rabbit kidney cell lysate
5. 40µg Hela cell lysate
Primary antibody: 0.5 µg/ml THETM α-tubulin Antibody[Biotin], mAb, Mouse (GenScript, A01545)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)

THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> Alpha Tubulin Antibody [Biotin], MAb, Mouse

Loading:
1. 60µg Hela cell lysate
2. 12µg Hela cell lysate
3. 2.4µg Hela cell lysate
Primary antibody: 0.5 µg/ml THETM α-tubulin Antibody[Biotin], mAb, Mouse (GenScript, A01545)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)


J Capecci., et al. Function of V-ATPase a Subunit Isoforms in Invasiveness of MCF10a and MCF10CA1a Human Breast Cancer Cells. J Biol Chem. 2013 Nov;288(45):32731-41.

Martinez-Espinosa PL, et al. Knockout of the BK β2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. J Gen Physiol. 2014Qct.;144(4):275-95

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Cat. No. A01545
Size
Price
100 ug $335.00
24 hour
 
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