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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM beta Actin Antibody [Biotin], mAb, Mouse

THETM beta Actin Antibody [Biotin], mAb, Mouse

Synonyms THE™ β-Actin Antibody [Biotin], mAb, Mouse;
Description GenScript THETM β-actin Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM β-Actin Antibody, mAb, Mouse (A00702) purified from mice ascites by protein A affinity column.
Host Species Mouse
Conjugation Biotin
Immunogen KLH-coupled synthetic peptide within residues 1-50 of human β-actin (Swiss Prot: P60709)
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Subclass Mouse IgG2a, k
Clone ID 2D1D10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody (100 μg) with 200 μl of deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
Specificity GenScript THETM β-actin Antibody[Biotin], mAb, Mouse recognizes Mouse, Rabbit, Chicken, Human, Hamster, Cow, Goat, Fish, Pig, and Monkey. The antibody has not yet been trested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA:0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> Beta Actin Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> Beta Actin Antibody [Biotin], MAb, Mouse

Loading:
1. 40μg pig muscle cell lysate
2. 40μg fish muscle cell lysate
3. 40μg CHO cell lysate
4. 40μg COS-7 cell lysate
5. 40μg rabbit kidney cell lysate
6. 40μg Hela cell lysate
7. 20μg Hela cell lysate
8. 10μg Hela cell lysate
Primary antibody: 0.5 µg/ml THETM β-actin Antibody[Biotin], mAb, Mouse (GenScript, A01546)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)


Cat. No.
Product Name
Size
Price
Quantity
A00702-100 THETM beta Actin Antibody, mAb, Mouse
100 ug
$175.00
A00702-40 THETM beta Actin Antibody, mAb, Mouse
40 ug
$69.00
A00702-200 THETM beta Actin Antibody, mAb, Mouse
200 ug
$311.00
A00730-200 THETM beta Actin Antibody [HRP], mAb, Mouse
200 ug
$333.00
A00885-40 α-Actin-1 Antibody, pAb, Rabbit
40 ug
$99.00
A00885-200 α-Actin-1 Antibody, pAb, Rabbit
200 ug
$349.00
A01203-40 β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01410-100 THETM alpha Tubulin Antibody, mAb, Mouse
100 ug
$225.00
A01410-40 THETM alpha Tubulin Antibody, mAb, Mouse
40 ug
$108.00
A01490 THETM alpha Tubulin Antibody [HRP], mAb, Mouse
100 ug
$335.00
A01627-40 Neuron specific β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01717-40 β-tubulin Antibody (2G7D4), mAb, Mouse
40 ug
$99.00
 


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Cat. No. A01546
Size
Price
100 ug $180.00
24 hour
 
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