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Catalog Products » Antibodies » Primary Antibodies » Alzheimer's Disease Antibody list » Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse

Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse

Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

Dot blot analysis of human β-Amyloid 1-40 and human β-Amyloid 1-42 using Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse (GenScript A01567).

Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse (GenScript A01567)
and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007).

Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

Cross-reactivity of Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse by Indirect ELISA.
General conditions:
1. Microplate was incubated with human β-Amyloid 32-40, human β-Amyloid 36-42, human β-Amyloid 1-40, or human β-Amyloid 1-42 respectively, followed by 3 washing cycles.
2. Incubation with Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

Synonyms β-amyloid Antibody (1-42), mAb, Mouse;
Description A number of mutations, identified in the gene encoding the β-amyloid precursor protein (βAPP), have been linked to early-onset Familial Alzheimers Disease. βAPP is cleaved sequentially by the proteolytic enzymes β-secretase and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42(43) and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides can bind to scavenger receptors and the receptor for advanced glycation end products. Aβ peptides are degraded either via a reuptake mechanism followed by endosomal degradation or by an extracellular insulin-degrading enzyme. Extracellular accumulation of Aβ leads to formation of aggregates, fibrils, and eventually amyloid deposits called neuritic plaques, a hallmark of Alzheimer’s disease. β-amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimers disease. GenScript Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and B-lymphocytes obtained from mouse immunized with a synthetic peptide corresponding to amino acids 36-42 of human β-Amyloid conjugated to KLH (Swiss Prot: P05067).
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute 40 µg lyophilized powder with 80 µl of deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Specificity GenScript Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse detects human β-Amyloid 1-42 and does not cross-react with human β-Amyloid 1-40.
Species Reactivity Human. Reactivity to other species is not tested yet.
Storage GenScript Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Dot blot: 0.1-1.0 µg/ml IHC: 1.0-5.0 µg/ml Other applications: user-optimized

  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse
  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

    Dot blot analysis of human β-Amyloid 1-40 and human β-Amyloid 1-42 using Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse (GenScript A01567).

  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse
  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

    Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse (GenScript A01567)
    and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007).

  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse
  • Human β-Amyloid 1-42 Antibody (2C2G5), MAb, Mouse

    Cross-reactivity of Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse by Indirect ELISA.
    General conditions:
    1. Microplate was incubated with human β-Amyloid 32-40, human β-Amyloid 36-42, human β-Amyloid 1-40, or human β-Amyloid 1-42 respectively, followed by 3 washing cycles.
    2. Incubation with Human β-Amyloid 1-42 Antibody (2C2G5), mAb, Mouse followed by 3 washing cycles.
    3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
    4. Colorimetric determination of bound peroxidase activity.


Cat. No.
Product Name
Price
A01566
Human β-Amyloid 1-40 Antibody (4A4F2), mAb, Mouse - 100 ug
$199.00
 
Kamalinia G., et al. Enhanced brain delivery of deferasirox-lactoferrin conjugates for iron chelation therapy in neurodegenerative disorders: in vitro and in vivo studies. Mol Pharm. 2013 Dec;10(12):4418-31.

Frederik Strang., et al. Amyloid Plaques Dissociate Pentameric to Monomeric C‐Reactive Protein: A Novel Pathomechanism Driving Cortical Inflammation in Alzheimer's Disease? Brain Pathol. 2012 May;22(3):337-46.

S Freire, et al. Photophysical study of Thioflavin T as fluorescence marker of amyloid fibrils. Dyes Pigm. 2014May;

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Cat. No. A01567
Size
Price
40 ug $99.00