Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ His Tag Antibody [FITC], mAb, Mouse
THE™ His Tag Antibody [FITC], MAb, Mouse

Flow cytometric analysis of transfected HEK293 cell
expressing His-tagged protein using THETM
Anti-His-tag [FITC] Monoclonal Antibody (GenScript, A01620;
shaded histogram) or with a negative control antibody (open
histogram).

THE™ His Tag Antibody [FITC], mAb, Mouse

GenScript THE™ His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
A01620
$410.00

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Overview
Specificity GenScript THE™ His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH
Conjugate Fluorescein (FITC)

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Immunocytochemistry/Immunofluorescence (ICC/IF) 0.1-2 µg/ml
Flow Cytometry 0.01-0.1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 6G2A9

Examples
  • THE™ His Tag Antibody [FITC], MAb, Mouse
  • THE™ His Tag Antibody [FITC], MAb, Mouse

    Flow cytometric analysis of transfected HEK293 cell
    expressing His-tagged protein using THETM
    Anti-His-tag [FITC] Monoclonal Antibody (GenScript, A01620;
    shaded histogram) or with a negative control antibody (open
    histogram).


Background
Target Background GenScript THE™ His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THE™ His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Synonyms THE™ His Tag Antibody [FITC], mAb, Mouse;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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