Catalog Products » Antibodies » Epitope Tag Antibodies » THETM BrdU Antibody, mAb, Mouse

THE™ BrdU Antibody, mAb, Mouse

*This product has been discontinued!*
Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
A01650
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Overview
Specificity The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T).
Host Species Mouse
Immunogen BrdU conjugated to KLH

Properties
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Purification Protein A affinity column
Clone ID 3E2D3

Background
Target Background Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.
GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
Synonyms Bromodeoxyuridine Antibody, mAb, Mouse; Bromo-deoxyuridine Antibody, mAb, Mouse

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