|Description||Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The OLLAS (SGFANELGPRLMGKC) epitope tag is a newly defined tag providing superior functionality on recombinant and fusion proteins without interfering with the biological activity of the tagged protein. GenScript OLLAS-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic OLLAS-tag epitope peptide.|
|Immunogen||KLH-coupled synthetic OLLAS-tag epitope peptide.|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript OLLAS-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing OLLAS epitope tag.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml
Other Applications: user optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
For more documents, please visit "Technical Support".