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Catalog Products » Antibodies » Epitope Tag Antibodies » OLLAS-tag Antibody, pAb, Rabbit

OLLAS-tag Antibody, pAb, Rabbit

Synonyms OLLAS-tag Antibody;
Description Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The OLLAS (SGFANELGPRLMGKC) epitope tag is a newly defined tag providing superior functionality on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.GenScript OLLAS-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic OLLAS-tag epitope peptide.
Host Species Rabbit
Conjugation Unconjugated
Immunogen KLH-coupled synthetic OLLAS-tag epitope peptide.
Purification Immunoaffinity chromatography

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript OLLAS-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing OLLAS epitope tag.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml
Other Applications: user optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

OLLAS-tag Antibody, PAb, Rabbit
OLLAS-tag Antibody, PAb, Rabbit

Western blot analysis of OLLAS tagged fusion protein using OLLAS-tag Antibody, pAb, Rabbit (GenScript, A01658, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 20 KD
Observed Size: 20 KD


Jerlström-Hultqvist J., et al. Hydrogenosomes in the diplomonad Spironucleus salmonicida Nat Commun. 2013 Sep;4:2493.

Jerlström-Hultqvist J., et al. Stable Transfection Of The Diplomonad Parasite Spironucleus Salmonicida. Eukaryot Cell. 2012 Nov;11(11):1353 - 1361.

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Cat. No. A01658
Size
Price
40 ug $99.00
24 hour
 
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