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Catalog Products » Antibodies » Epitope Tag Antibodies » SUMO-tag Antibody(4G11E9), mAb, Mouse

SUMO-tag Antibody(4G11E9), mAb, Mouse

SUMO-tag Antibody(4G11E9), MAb, Mouse

Western blot analysis of SUMO-tagged fusion protein using SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 62 kD
Observed Size: 62 kD

SUMO-tag Antibody(4G11E9), MAb, Mouse

Western Blot of SUMO protein with SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693)

Lane 1: 20 ng SUMO protein
Lane 2: 10 ng SUMO protein
Lane 3: 5 ng SUMO protein

Primary Antibody:
SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693), 1 µg/ml

Secondary Antibody:
Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific, 0.2 μg/ml

Predicted Size: 11 kD
Observed Size: 18 kD

Specificity GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse specifically reacts with fusion proteins containing SUMO epitope tags.
Host Species Mouse
Immunogen Purified recombinant yeast SUMO protein
(DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDG             IRIQADQAPEDLDMEDNDIIEAHREQIGG)
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.5-1 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 4G11E9

  • SUMO-tag Antibody(4G11E9), MAb, Mouse
  • SUMO-tag Antibody(4G11E9), MAb, Mouse

    Western blot analysis of SUMO-tagged fusion protein using SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.
    Predicted Size: 62 kD
    Observed Size: 62 kD

  • SUMO-tag Antibody(4G11E9), MAb, Mouse
  • SUMO-tag Antibody(4G11E9), MAb, Mouse

    Western Blot of SUMO protein with SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693)

    Lane 1: 20 ng SUMO protein
    Lane 2: 10 ng SUMO protein
    Lane 3: 5 ng SUMO protein

    Primary Antibody:
    SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693), 1 µg/ml

    Secondary Antibody:
    Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific, 0.2 μg/ml

    Predicted Size: 11 kD
    Observed Size: 18 kD


Target Background SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. The SUMO system can be used effectively in both prokaryotic and eukaryotic systems. GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is suitable for detecting fusion proteins that contain a SUMO-tag.GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with recombinant yeast SUMO protein.
Synonyms SUMO Antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.






For more documents, please visit "Technical Support".

Cat. No. A01693
Size Price
40 μg $99.00