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Catalog Products » Other Products » GFP Antibody, pAb, Chicken

GFP Antibody, pAb, Chicken

GFP Antibody, PAb, Chicken

Western blot analysis of GFP tagged fusion protein using GFP Antibody, pAb, Chicken (GenScript, A01694, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Chicken IgG.
Predicted Size: 27 kD
Observed Size: 27 kD

GFP Antibody, PAb, Chicken

Specificity analysis of GFP Antibody, pAb, Chicken (GenScript, A01694) by Western blot, using a variety of variants of GFP protein
such as eGFP and AcGFP1, and variety of variants of RFP protein such as tdTomato, mOrange, DsRed, DeRed2, and mCherry.
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Chicken IgG.

GFP Antibody, PAb, Chicken

Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
1: Cell lysates containing GFP tagged fusion protein as input control.
2: Immunoprecipitates of the cell lysates incubated with Chicken IgY Control (GenScript, A01010) as negative control.
3: Immunoprecipitates of the cell lysates incubated with GFP Antibody, pAb, Chicken (GenScript, A01694).

Synonyms Green Fluorescent Protein antibody;
Description Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.
GenScript GFP Antibody, pAb, Chicken is developed in chicken using purified recombinant full-length GFP protein.
Host Species Chicken
Conjugation Unconjugated
Immunogen purified recombinant full-length GFP protein
Purification Immunoaffinity chromatography

Subclass IgY
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
Other Applications: user optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

GFP Antibody, PAb, Chicken
GFP Antibody, PAb, Chicken

Western blot analysis of GFP tagged fusion protein using GFP Antibody, pAb, Chicken (GenScript, A01694, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Chicken IgG.
Predicted Size: 27 kD
Observed Size: 27 kD

GFP Antibody, PAb, Chicken
GFP Antibody, PAb, Chicken

Specificity analysis of GFP Antibody, pAb, Chicken (GenScript, A01694) by Western blot, using a variety of variants of GFP protein
such as eGFP and AcGFP1, and variety of variants of RFP protein such as tdTomato, mOrange, DsRed, DeRed2, and mCherry.
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Chicken IgG.

GFP Antibody, PAb, Chicken
GFP Antibody, PAb, Chicken

Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
1: Cell lysates containing GFP tagged fusion protein as input control.
2: Immunoprecipitates of the cell lysates incubated with Chicken IgY Control (GenScript, A01010) as negative control.
3: Immunoprecipitates of the cell lysates incubated with GFP Antibody, pAb, Chicken (GenScript, A01694).


Cat. No.
Product Name
Size
Price
Quantity
A01388-40 GFP Antibody, pAb, Rabbit
40 ug
$99.00
A01694-40 GFP Antibody, pAb, Chicken
40 ug
$99.00
A01703-40 GFP Antibody, pAb, Goat
40 ug
$99.00
A01704-40 THETM GFP Antibody, pAb, Rabbit
40 ug
$198.00
A01704-100 THETM GFP Antibody, pAb, Rabbit
100 ug
$412.00
A01704-200 THETM GFP Antibody, pAb, Rabbit
200 ug
$741.00
A01694-100 GFP Antibody, pAb, Chicken
100 ug
$199.00
A01703-100 GFP Antibody, pAb, Goat
100 ug
$199.00
 
JC McIntyre., et al. Gene Therapy Rescues Cilia Defects And Restores Olfactory Function In A Mammalian Ciliopathy Model. Nat Med. 2012 Sep;18(9):1423 - 8.

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For more documents, please visit "Technical Support".

Cat. No. A01694
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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