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Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ GFP Antibody, pAb, Rabbit

THE™ GFP Antibody, pAb, Rabbit

THE™ GFP Antibody, PAb, Rabbit

Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
The assay was performed with GFP-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

THE™ GFP Antibody, PAb, Rabbit

Specificity analysis of THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) by Western blot, using a variety of variants of GFP protein
such as eGFP and AcGFP1, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry, tdTomato and mOrange.
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
1: Cell lysates containing GFP tagged fusion protein as input control.
2: Immunoprecipitates of the cell lysates incubated with Rabbit IgG Control (GenScript, A01008) as negative control.
3: Immunoprecipitates of the cell lysates incubated with THETM GFP Antibody, pAb, Rabbit (GenScript, A01704).

THE™ GFP Antibody, PAb, Rabbit

Flow cytometric analysis of non-transfected or GFP fusion gene transfeced HEK293 cells
using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) (black and red, respectively).
The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Immunocytochemistry/Immunofluorescence analysis of GFP fusion gene transfeced HEK293 cells
using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) and Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008)
The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
The assay was performed with GFP-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Specificity GenScript THE™ GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.
Host Species Rabbit
Immunogen purified recombinant full-length GFP protein
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 0.2-1 µg/ml
Immunoprecipitation 1-5 µg/ml
Flow cytometry 1-3 µg for 1 x 106 cells
ICC/IF 1-3 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
    The assay was performed with GFP-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Specificity analysis of THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) by Western blot, using a variety of variants of GFP protein
    such as eGFP and AcGFP1, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry, tdTomato and mOrange.
    The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
    1: Cell lysates containing GFP tagged fusion protein as input control.
    2: Immunoprecipitates of the cell lysates incubated with Rabbit IgG Control (GenScript, A01008) as negative control.
    3: Immunoprecipitates of the cell lysates incubated with THETM GFP Antibody, pAb, Rabbit (GenScript, A01704).

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Flow cytometric analysis of non-transfected or GFP fusion gene transfeced HEK293 cells
    using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) (black and red, respectively).
    The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Immunocytochemistry/Immunofluorescence analysis of GFP fusion gene transfeced HEK293 cells
    using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) and Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008)
    The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
    The assay was performed with GFP-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Target Background Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.GenScript THE™ GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.
Synonyms Green Fluorescent Protein antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
A01704-100
THE™ GFP Antibody, pAb, Rabbit - 100 μg
$412.00
A01388-40
GFP Antibody, pAb, Rabbit - 40 μg
$99.00
A00185-40
cGFP-tag Antibody, mAb, Mouse - 40 μg
$99.00
 
Anirban Dasgupta, et al. Molecular characterization of substrate-induced ubiquitin transfer by UBR7-PHD finger, a newly identified histone H2BK120 ubiquitin ligase. FEBS J. (2021-11)

Tong Zhu, et al. Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana. Mol Plant Pathol. (2021-10)

Dokshin GA, et al. GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome Stability. Developmental Cell. (2020-01)

ZhangJianjian, et al. DENN domain-containing protein FAM45A regulates the homeostasis of late/multivesicular endosomes. Biochim Biophys Acta Mol Cell Res. (2019-05)

Nagashima Y1, et al. Multiple quality control mechanisms in the ER and TGN determine subcellular dynamics and salt-stress tolerance function of KORRIGAN 1. Plant Cell. (2019)

Ishidate T, et al. ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals. Molecular Cell. (2018-05)

LiuJun, et al. A Tyrosine Phosphorylation Cycle Regulates Fungal Activation of a Plant Receptor Ser/Thr Kinase. Cell Host Microbe. (2018)

Al Abdallah Qusai, et al. C-terminus Proteolysis and Palmitoylation Cooperate for Optimal Plasma Membrane Localization of RasA in . Front Microbiol. (2018)

Renshaw H, et al. The tail domain of the Aspergillus fumigatus class V myosin MyoE orchestrates septal localization and hyphal growth. J Cell Sci. (2018)

Nakagawa Y, et al. Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin. PLoS One. (2016-02)

Kimberly J. Krager, et al. A Novel Biotinylated Lipid Raft Reporter For Electron Microscopic Imaging Of Plasma Membrane Microdomains. J Lipid Res. (2012-10)

Kadauke S, et al. Tissue-specific mitotic bookmarking by hematopoietic transcription factor GATA1. Cell. (2012-08)

You can visit "Citations Database" for more citations.




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Cat. No. A01704
Size Price
100 μg $412.00