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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM GFP Antibody, pAb, Rabbit

THE™ GFP Antibody, pAb, Rabbit

THE™ GFP Antibody, PAb, Rabbit

Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
The assay was performed with GFP-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

THE™ GFP Antibody, PAb, Rabbit

Specificity analysis of THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) by Western blot, using a variety of variants of GFP protein
such as eGFP and AcGFP1, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry, tdTomato and mOrange.
The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
1: Cell lysates containing GFP tagged fusion protein as input control.
2: Immunoprecipitates of the cell lysates incubated with Rabbit IgG Control (GenScript, A01008) as negative control.
3: Immunoprecipitates of the cell lysates incubated with THETM GFP Antibody, pAb, Rabbit (GenScript, A01704).

THE™ GFP Antibody, PAb, Rabbit

Flow cytometric analysis of non-transfected or GFP fusion gene transfeced HEK293 cells
using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) (black and red, respectively).
The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Immunocytochemistry/Immunofluorescence analysis of GFP fusion gene transfeced HEK293 cells
using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) and Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008)
The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

THE™ GFP Antibody, PAb, Rabbit

Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
The assay was performed with GFP-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Host Species Rabbit
Conjugation Unconjugated
Immunogen purified recombinant full-length GFP protein
Purification Immunoaffinity chromatography
Specificity GenScript THETM GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other Applications: user optimized

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
    The assay was performed with GFP-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Specificity analysis of THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) by Western blot, using a variety of variants of GFP protein
    such as eGFP and AcGFP1, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry, tdTomato and mOrange.
    The signal was developed with IRDyeTM 800 Conjugated Rabbit Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Western blot analysis of cell lysates containing GFP tagged fusion protein and its immunoprecipitates.
    1: Cell lysates containing GFP tagged fusion protein as input control.
    2: Immunoprecipitates of the cell lysates incubated with Rabbit IgG Control (GenScript, A01008) as negative control.
    3: Immunoprecipitates of the cell lysates incubated with THETM GFP Antibody, pAb, Rabbit (GenScript, A01704).

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Flow cytometric analysis of non-transfected or GFP fusion gene transfeced HEK293 cells
    using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) (black and red, respectively).
    The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Immunocytochemistry/Immunofluorescence analysis of GFP fusion gene transfeced HEK293 cells
    using THETM GFP Antibody, pAb, Rabbit (GenScript, A01704) and Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008)
    The signal was developed with R-PE conjugated Goat Anti-Rabbit IgG.

  • THE™ GFP Antibody, PAb, Rabbit
  • THE™ GFP Antibody, PAb, Rabbit

    Comparison of THETM GFP Antibody, pAb, Rabbit (Left: GenScript, A01704, 0.2 µg/ml) with Rabbit Anti-GFP pAb (Right: Competitor, ab290, 1:5000) by Western blot.
    The assay was performed with GFP-tagged fusion protein.
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Synonyms Green Fluorescent Protein antibody;
Target Background Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.
GenScript THETM GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.

Cat. No. Product Name Quantity Price
A01704-100
THE™ GFP Antibody, pAb, Rabbit - 100 μg
$412.00
A01388-40
GFP Antibody, pAb, Rabbit - 40 μg
$99.00
A00185-40
cGFP-tag Antibody, mAb, Mouse - 40 μg
$99.00
 




For more documents, please visit "Technical Support".

Cat. No. A01704
Size Price
100 μg $412.00