Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Figure 1. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a flow cytometric analysis of non-transfected or Strep II gene-transfected CHO cells (black and blue, respectively) using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Figure 2. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in an immunocytochemical/immunofluorescence analysis of Strep II tagged protein-transfected HEK293 cells using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Figure 3. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a Western blot analysis of C-terminal Strep II tagged fusion protein using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Figure 4. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II in immunoprecipitates was analyzed using Western blotting. Immunoprecipitates from Strep II-tagged protein-transfected HEK293 cell lysates using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the non-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Figure 5. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for N-terminal Strep II in an N-terminal Strep II tagged fusion was analyzed by Western blotting using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes NWSHPQFEK tags localized at the N-terminal and C-terminal region of Strep II-tagged fusion proteins. 
A01732
$330.00

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Overview
Specificity THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes NWSHPQFEK tags localized at the N-terminal and C-terminal region of Strep II-tagged fusion proteins. 
Host Species Mouse
Immunogen NWSHPQFEK peptide conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot  0.1-0.5 µg/ml
Immunoprecipitation (IP)  1-5 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)  1-3 µg/ml
ELISA  0.001-0.002 µg/ml
Flow Cytometry  1 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. 
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 5A9F9
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Figure 1. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a flow cytometric analysis of non-transfected or Strep II gene-transfected CHO cells (black and blue, respectively) using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Figure 2. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in an immunocytochemical/immunofluorescence analysis of Strep II tagged protein-transfected HEK293 cells using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Figure 3. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a Western blot analysis of C-terminal Strep II tagged fusion protein using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Figure 4. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II in immunoprecipitates was analyzed using Western blotting. Immunoprecipitates from Strep II-tagged protein-transfected HEK293 cell lysates using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
    Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
    Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
    Lane 3: Immunoprecipitates of the non-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Figure 5. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for N-terminal Strep II in an N-terminal Strep II tagged fusion was analyzed by Western blotting using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.


Background
Target Background Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It is widely used in numerous molecular biological protocols due to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
Synonyms THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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