THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
| Specificity | THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | NWSHPQFEK peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||||||
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 5A9F9 |
Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
| Target Background | Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins. |
| Synonyms | THE™ NWSHPQFEK Tag Antibody,mAb,Mouse |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01742-100 |
THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 100 μg |
$349.00 |
|
A01737-100 |
THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse - 100 μg |
$349.00 |
|
A01736-100 |
THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse - 100 μg |
$349.00 |
|
A01732-100 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 100 μg |
$319.00 |
|
A01732-200 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 200 μg |
$579.00 |
|
A00875-40 |
NWSHPQFEK Antibody [HRP], pAb, Rabbit - 40 μg |
$99.00 |
|
A00626-40 |
NWSHPQFEK Antibody, pAb, Rabbit - 40 μg |
$99.00 |
Tucker K, et al. Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution. Nat Struct Mol Biol. (2019)
PauVictor, et al. Crystal structure of an inactivated mutant mammalian voltage-gated K channel. Nat. Struct. Mol. Biol. (2017)
XuPeng, et al. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLoS Pathog. (2017)
Wei L,, Zhao X., et al. A new MCM modification cycle regulates DNA replication initiation. Nat Struct Mol Biol. (2016)
You can visit "Citations Database" for more citations.
PauVictor, et al. Crystal structure of an inactivated mutant mammalian voltage-gated K channel. Nat. Struct. Mol. Biol. (2017)
XuPeng, et al. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLoS Pathog. (2017)
Wei L,, Zhao X., et al. A new MCM modification cycle regulates DNA replication initiation. Nat Struct Mol Biol. (2016)
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A01732 | ||
|---|---|---|
| Size | Price | |
| 100 μg | $319.00 | |
| 200 μg | $579.00 | |
