Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Synonyms | THETM NWSHPQFEK Tag Antibody,mAb,Mouse |
Description | Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins. |
Host Species | Mouse |
Conjugation | Unconjugated |
Immunogen | NWSHPQFEK peptide conjugated to KLH |
Fusion Partner | Fusion of SP2/0-Ag14 myeloma and B-lymphocytes |
Purification | Protein A affinity column |
Subclass | IgG1 Κ |
Clone ID | 5A9F9 |
Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Reconstitution | Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. |
Specificity | THETM NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins. |
Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/ml Immunoprecipitation: 1-5 µg/ml ICC/IF: 1-3 µg/ml ELISA: 0.001-0.002 µg/ml Flow cytometry: 1 µg/ml |
Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
Cat. No. | Product Name | Quantity | Price |
---|---|---|---|
A01742-100 |
THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 100 ug |
$349.00 |
|
A01737-100 |
THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse - 100 ug |
$349.00 |
|
A01736-100 |
THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse - 100 ug |
$349.00 |
|
A01732-100 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 100 ug |
$319.00 |
|
A01732-200 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 200 ug |
$579.00 |
|
A00875-40 |
NWSHPQFEK Antibody [HRP], pAb, Rabbit - 40 ug |
$99.00 |
|
A00626-40 |
NWSHPQFEK Antibody, pAb, Rabbit - 40 ug |
$99.00 |
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