Western blot analysis of C-terminal V5 tagged fusion protein using THETM V5 Tag Antibody [HRP], mAb, Mouse (A01733, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
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| Specificity | THE™ V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | V5 tag peptide conjugated to KLH |
| Conjugate | Horseradish peroxidase (HRP) |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.01% thimerosal. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2a,κ |
| Clonality | Monoclonal |
| Clone ID | 4C12E11 |
Western blot analysis of C-terminal V5 tagged fusion protein using THETM V5 Tag Antibody [HRP], mAb, Mouse (A01733, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
| Target Background | V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.GenScript THE™ V5 Tag Antibody [HRP], mAb, Mouse is THE™ V5 Tag Antibody, mAb, Mouse (A01724) conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins. |
| Synonyms | THE™ V5 Tag Antibody [HRP], mAb, Mouse ; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
