THE™ PEG Antibody, mAb, Mouse

GenScript’s THE™ PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.

Price	$154.00
Cat. No.	A01795-100
Size	100 μg 500 μg Buy in bulk
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript’s THE™ PEG Antibody, mAb, Mouse has high affinity for the PEG backbone. The antibody binds to the variety of PEG such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.
Host Species	Mouse
Immunogen	PEG conjugated to KLH
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.

Application	Recommended Usage
Sandwich ELISA	Capture: 5-10 µg/ml Detection: 0.1-1.0 µg/ml
Competitive ELISA	User-optimized
Western Blot	0.1-1.0 µg/ml
Immunohistochemistry (IHC)	10-15 µg/ml

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Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. For long-term storage, adding glycerol to a final concentration of 50% (v/v) in the reconstituted antibody solution is recommended.
Storage Instructions	The product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Mouse IgM
Clonality	Monoclonal
Clone ID	5E10E9
Note	Some reagents such as Tween-20, Triton X-100 and NP-40 interfere with the PEG antibody interactions due to their structural similarity to PEG. Antibody solution should not contain such reagents in the assay of PEGylated molecules. GenScript can customize this product per customer's request including product size, buffer components, etc.

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Background

Target Background

PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalently couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs. PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half-life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Polyethylene glycol (PEG) antibody is a useful tool for the detection of PEGylated molecules.

Synonyms

Anti-Polyethylene glycol antibody, mAb, Mouse

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Parul Sirohi, et al. All-PEG-Like Block Copolymers Self-Assemble into Stealth Nanocarriers for Drug Delivery. Advanced Science. (2026-01)
2. LA Mejíamanzano, et al. Covalent immobilization of antibodies for the preparation of immunoaffinity chromatographic supports. SEP SCI TECHNOL. (2016-04)