×
Catalog Products » Antibodies » THE™ Elite Antibodies » THETM PEG Antibody [Biotin], mAb, Mouse

THETM PEG Antibody [Biotin], mAb, Mouse

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

ELISA analysis of PEG40 and PEG20 using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100).

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

Detection of variety of PEGs using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100, 1 µg/ml).
The test result showed GenScript PEG Antibody could detect PEGs with different molecular weight and
had reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12).

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

Western blot analysis of PEGylated drug (Pegasys, Peginterferon Alfa 2A) and Non-PEGylated Interferon
Alfa 2A protein using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100, 0.2 µg/ml).

A PEGylated drug (Pegasys, Peginterferon Alfa 2A)
B Interferon Alfa 2A protein

Synonyms PEG Antibody [Biotin]; PEG Monoclonal Antibody [Biotin]; Anti PEG Antibody [Biotin]; Anti PEG Monoclonal Antibody [Biotin]; Anti Polyethylene glycol Antibody [Biotin]; Polyethylene glycol Antibody [Biotin]; Polyethylene glycol Monoclonal Antibody [Biotin];
Description PEG (Polyethylene glycol) is a polyether compound with many applications from industrial manufacturing to medicine. PEGylation is a technology that covalently couples non-toxic, hydrophilic polyethylene glycol (PEG) to the drug. It is an FDA-approved method for the delivery of protein drugs. PEG modification can reduce the drug immunogenicity and antigenicity. PEGylated drug decelerates renal excretion, improves stability towards proteolysis and increases its half life in blood. Accurate and sensitive quantification of PEG conjugates is important for PEG conjugated product development and pharmaceutical study. Biotin conjugated PEG Antibody is a useful tool for the detection of PEGylated molecules.
GenScript THETM PEG Antibody [Biotin], mAb, Mouse is PEG antibody conjugated with biotin.
Host Species Mouse
Conjugation Biotin
Immunogen PEG conjugated to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Affinity chromatography

Subclass IgM
Clone ID 5E10E9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. For long-term storage, we suggest adding glycerol to 50% to the reconstituted antibody.
Specificity GenScript THETM PEG Antibody [Biotin], mAb, Mouse detects PEG backbone. The product can detect the variety of PEGs such as PEG40K, PEG20K, PEG5K, PEG12, PEGylated drugs and PEG conjugates.
Storage GenScript THETM PEG Antibody [Biotin], mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20 °C or below. The reconstituted product can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA Detector: 0.1-1.0 µg/ml
Western Blot: 0.1-1.0 µg/ml
ELISA Capturer: customer optimized
Competitive ELISA: customer optimized
Double Antigen Bridging ELISA: customer optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

ELISA analysis of PEG40 and PEG20 using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100).

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

Detection of variety of PEGs using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100, 1 µg/ml).
The test result showed GenScript PEG Antibody could detect PEGs with different molecular weight and
had reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12).

THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> PEG Antibody [Biotin], MAb, Mouse

Western blot analysis of PEGylated drug (Pegasys, Peginterferon Alfa 2A) and Non-PEGylated Interferon
Alfa 2A protein using THETM PEG Antibody [Biotin], mAb, Mouse (Cat.No. A01796-100, 0.2 µg/ml).

A PEGylated drug (Pegasys, Peginterferon Alfa 2A)
B Interferon Alfa 2A protein


Cat. No.
Product Name
Size
Price
Quantity
A01795-100 THETM PEG Antibody, mAb, Mouse
100 ug
$144.00
A01795-500 THETM PEG Antibody, mAb, Mouse
500 ug
$645.00
 


Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01796
Size
Price
100 ug $158.00
24 hour
 
*
*
*
*