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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM ADP Antibody, mAb, Mouse

THETM ADP Antibody, mAb, Mouse

Synonyms ADP Antibody, mAb, Mouse
Description Adenosine diphosphate (ADP) is the product of adenosine triphosphate (ATP) dephosphorylation by ATPases. It is an important intermediate in cellular metabolism as the partially dephosphorylated form of ATP. ADP is 5′-adenylic acid with an additional phosphate group attached through a pyrophosphate bond. Its molecular formula is C10H15N5O10P2 with the molecular weight of 427.201 g/mol. ADP immunoassay is a useful method for screening the activity of kinases and other ATPases. An ADP antibody is a key reagent for ADP immunoassays such as ELISA and TR-FRET.
GenScript THE™ ADP Antibody, mAb, Mouse is produced from the hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with ADP conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Immunogen ADP conjugated to KLH
Purification Protein A affinity column

Subclass IgG1,κ
Clone ID 9B2B9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/ml.
Specificity The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding (EC50). The cross-reactivity data obtained in a competitive ELISA system is as follows:

Compound

Cross reactivity with ADP%

ADP

100

GDP

53.33

ATP

7.11

GTP

4.37

cAMP

0.47

CTP

0.03

cGMP

0.01

GMP

0.0085

cIMP

0.005

AMP

< 0.0001

UMP

< 0.0001

cTMP

< 0.0001



Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Competitive ELISA: 0.001-0.01 µg/ml.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> ADP Antibody, MAb, Mouse
THE<sup>TM</sup> ADP Antibody, MAb, Mouse

Competitive ELISA of ADP standard curve using THETM ADP Antibody, mAb, Mouse (GenScript, A01799)


Cat. No.
Product Name
Size
Price
Quantity
A01316-40 ADP Antibody, pAb, Rabbit
40 μl
$99.00
A01316-200 ADP Antibody, pAb, Rabbit
200 μl
$349.00
A01817-100 Phosphotyrosine Antibody (5E10), mAb, Mouse
100 ug
$199.00
A01818-100 Phosphotyrosine Antibody (18E10), mAb, Mouse
100 ug
$199.00
A01819-100 THETM Phosphotyrosine Antibody (E10)plus
100 ug
$265.00
 


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Cat. No. A01799
Size
Price
40 ug $140.00
100 ug $350.00
24 hour
 
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