Catalog Products » Antibodies » Secondary Antibodies » Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb
Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

Western blot of IP samples with A01827 or conventional secondary antibodies.
Loading:
Lane 1,3: Protein A beads (40 µl) + Glutamine Synthetase Antibody, pAb, Rabbit (10 µg) + Mouse brain lysate (200 µg)
Lane 2,4: Mouse brain lysate (20 µg)

Primary antibody:
Lane 1~4: Glutamine Synthetase Antibody, pAb, Rabbit

Secondary antibody:
Lane 1,2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
Lane 3,4: Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins), 1:5,000

Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

Western blot of reduced and non-reduced rabbit IgG with A01827.
Loading:
Lane 1: Rabbit IgG (Reduced), 200 ng
Lane 2: Rabbit IgG (Non-reduced), 200 ng
We recommend loading no more than 200 ng of purified sample per lane

Detection antibody:
Lane 1~2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000

Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb

GenScript Anti-Rabbit IgG Antibody (M205) only reacts with native rabbit IgG and does not bind to the denatured or reduced rabbit IgG heavy chain or light chain. The monoclonal antibody has no cross-reactivity with mouse, rat, bovine, goat, or sheep immunoglobulins and limited cross-reactivity with human immunoglobulins.
A01827
$92.00

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Overview
Specificity GenScript Anti-Rabbit IgG Antibody (M205) only reacts with native rabbit IgG and does not bind to the denatured or reduced rabbit IgG heavy chain or light chain. The monoclonal antibody has no cross-reactivity with mouse, rat, bovine, goat, or sheep immunoglobulins and limited cross-reactivity with human immunoglobulins.
Host Species Mouse
Immunogen Rabbit IgG(H&L)
Species Reactivity Rabbit
Conjugate Horseradish peroxidase (HRP)

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Optimal working dilutions must be determined by the end user.
Application Recommended Usage
Western Blot 1:5,000 -1:20,000
ELISA 1:5,000 -1:20,000
Immunohistochemistry (IHC) User-optimized

Properties
Form Liquid
Storage Buffer Supplied in PBS (pH 7.4), containing 1% BSA, 50% glycerol and 0.01% thimerosal.
Concentration Not applicable
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID M205

Examples
  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb
  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb
  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

    Western blot of IP samples with A01827 or conventional secondary antibodies.
    Loading:
    Lane 1,3: Protein A beads (40 µl) + Glutamine Synthetase Antibody, pAb, Rabbit (10 µg) + Mouse brain lysate (200 µg)
    Lane 2,4: Mouse brain lysate (20 µg)

    Primary antibody:
    Lane 1~4: Glutamine Synthetase Antibody, pAb, Rabbit

    Secondary antibody:
    Lane 1,2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
    Lane 3,4: Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins), 1:5,000

  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb
  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb

    Western blot of reduced and non-reduced rabbit IgG with A01827.
    Loading:
    Lane 1: Rabbit IgG (Reduced), 200 ng
    Lane 2: Rabbit IgG (Non-reduced), 200 ng
    We recommend loading no more than 200 ng of purified sample per lane

    Detection antibody:
    Lane 1~2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000


Background
Target Background Generally, goat or donkey anti-rabbit IgG polyclonal antibodies are used as secondary antibodies to detect rabbit primary antibodies. However, since polyclonal antibodies recognize many epitopes, the background and cross reactivity in many assays is usually high, making results hard to interpret. To minimize background, cross reactivity and improve the lot-to-lot consistency, a unique Anti-Rabbit IgG Antibody (M205) has been developed to use as a secondary antibody. Simply substitute conventional HRP or fluorescent reagents for unparalleled clarity in results and high quality results in immunoprecipitation, Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Anti-Rabbit IgG Antibody (M205) only recognizes native rabbit primary IgG antibodies. This antibody does not react with denatured or reduced rabbit IgG heavy or light chain. Immunoprecipitation and Western blots often suffer from primary antibody heavy and light chain contamination at approximately 50 and 25 kDa, which is caused by poor specificity of conventional polyclonal secondary antibodies derived from goat or other species.
Synonyms Anti-Rabbit IgG [HRP]

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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