![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb](/product/documents/down?doc_name=antibody02.jpg&file=scm_files/productFile_notes/2025/03/25/antibody02.jpg)
![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb](/product/documents/down?doc_name=2015121501.jpg&file=scm_files/productFile_notes/2016/11/30/4f6234cc2d20e4ff2d291d3109f42a57.jpg)
![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb](/product/documents/down?doc_name=A01827_IP.jpg&file=scm_files/productFile_notes/2023/04/24/6f12f3a8-b750-464f-89c5-27bf4546169c.jpg)
Western blot of IP samples with A01827 or conventional secondary antibodies.
Loading:
Lane 1,3: Protein A beads (40 µl) + Glutamine Synthetase Antibody, pAb, Rabbit (10 µg) + Mouse brain lysate (200 µg)
Lane 2,4: Mouse brain lysate (20 µg)
Primary antibody:
Lane 1~4: Glutamine Synthetase Antibody, pAb, Rabbit
Secondary antibody:
Lane 1,2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
Lane 3,4: Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins), 1:5,000
![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb](/product/documents/down?doc_name=A01827_WB.jpg&file=scm_files/productFile_notes/2023/04/24/9c40914e-1c84-4171-bfc5-93eb0e3c1e1e.jpg)
Western blot of reduced and non-reduced rabbit IgG with A01827.
Loading:
Lane 1: Rabbit IgG (Reduced), 200 ng
Lane 2: Rabbit IgG (Non-reduced), 200 ng
We recommend loading no more than 200 ng of purified sample per lane
Detection antibody:
Lane 1~2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
![Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb](/product/documents/down?doc_name=A01827_IP_2.jpg&file=scm_files/productFile_notes/2024/08/05/59f7903a-9acc-476c-9203-1e3e9d211cdc.jpg)
Comparison of A01827 and Competitor Antibodies in Western Blot Analysis of IP Samples.
Loading:
Lanes 1,2,3: 40 µl Protein A beads + 10 µg Glutamine Synthetase Antibody, pAb, Rabbit + 200 µg Mouse brain lysate
Primary antibody:
Lanes 1,2,3: Glutamine Synthetase Antibody, pAb, Rabbit
Secondary antibody:
Lane 1: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), diluted 1:5,000
Lane 2: Competitor antibody (Conformation Specific), diluted 1:2,000
Lane 3: Conventional peroxidase-conjugated Goat Anti-RABBIT IgG (H&L) secondary antibody, diluted 1:5,000
Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb
A01827 | |
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