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Catalog Products » Other Products » Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb

Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb

Description Generally, goat or donkey anti-rabbit IgG polyclonal antibodies are used as secondary antibodies to detect rabbit primary antibodies. However, since polyclonal antibodies recognize many epitopes, the background and cross reactivity in many assays is usually high, making results hard to interpret. To minimize background, cross reactivity and improve the lot-to-lot consistency, a unique Anti-Rabbit IgG Antibody (M205) has been developed to use as a secondary antibody. Simply substitute conventional HRP or fluorescent reagents for unparalleled clarity in results and high quality results in immunoprecipitation, Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Anti-Rabbit IgG Antibody (M205) only recognizes native rabbit primary IgG antibodies. This antibody does not react with denatured or reduced rabbit IgG heavy or light chain. Immunoprecipitation and Western blots often suffer from primary antibody heavy and light chain contamination at approximately 50 and 25 kDa, which is caused by poor specificity of conventional polyclonal secondary antibodies derived from goat or other species.
Host Species Mouse
Antigen Species Rabbit
Conjugation Horseradish Peroxidase (HRP)
Immunogen Rabbit IgG(H&L)
Purification Protein A affinity column
Key Features GenScript Anti-Rabbit IgG Antibody (M205) only reacts with native rabbit IgG and does not bind to the denatured or reduced rabbit IgG heavy chain or light chain. The monoclonal antibody has no cross-reactivity with mouse, rat, bovine, goat, or sheep immunoglobulins and limited cross-reactivity with human immunoglobulins.

Subclass IgG1,κ
Concentration Supplied in PBS (pH 7.4), 10 mg/ml BSA and 50% glycerol
Specificity GenScript Anti-Rabbit IgG Antibody (M205) only reacts with native rabbit IgG and does not bind to the denatured or reduced rabbit IgG heavy chain or light chain. The monoclonal antibody has no cross-reactivity with mouse, rat, bovine, goat, or sheep immunoglobulins and limited cross-reactivity with human immunoglobulins.
Species Reactivity Rabbit
Storage GenScript Anti-Rabbit IgG Antibody should be stored at -20°C

Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blotting:1:5,000 -1:20,000
ELISA:1:5,000 -1:20,000
Immunohistochemistry:user-optimized
Optimal working dilutions must be determined by the end user.

  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb
  • Mouse Anti-Rabbit IgG Antibody (M205) [HRP], MAb




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Cat. No. A01827
Size
Price
200 ul $79.00