• English
  • Sign In
  • Contact Us
×
Catalog Products » Antibodies » Primary Antibodies » Immune Checkpoint Antibodies » Human PD-1 Antibody (PD1.H5), mAb, Mouse

Human PD-1 Antibody (PD1.H5), mAb, Mouse

Human PD-1 Antibody (PD1.H5), MAb, Mouse

ELISA binding of human PD-1 antibody PD1.H5 (GenScript, A01828) with Human PD-1 recombinant protein (Z03370, PD 1 Fc Chimera, Human).
Coating antigen: PD-1-Fc, 1 µg/ml.
PD-1 antibody dilution start from 1000 ng/ml, EC50= 4.29 ng/ml

Human PD-1 Antibody (PD1.H5), MAb, Mouse

ELISA blocking of human PD-1 antibody PD1.H5 (GenScript, A01828) against Human PD-L1 recombinant protein (Z03371, PD 1 Fc Chimera, Human) binding with Human PD-1 recombinant protein (Z03370, PD 1 Fc Chimera, Human).
Coating antigen: PD-1-Fc, 1 µg/ml.
PD-L1-Fc final concentration: 0.5 µg /ml
PD-1 antibody dilution start from 50 µg/ml, IC50= 15.5 µg/ml

Human PD-1 Antibody (PD1.H5), MAb, Mouse

Flow cytometric analysis of CHO-K1/PD1 stable cell expressing PD-1 (GenScript, M00529, Red) and CHO negative control cell (Black) binding with Human PD-1 Antibody PD1.H5 (GenScript, A01828)
Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Human PD-1 Antibody (PD1.H5), MAb, Mouse

FACS ligand blocking tests of Human PD-1 Antibody PD1.H5 (GenScript, A01828) blocks the binding of human PD-1 cell line (GenScript, M00529) with Human PD-L1 (Z03371, PD L1 Fc Chimera, Human. Red) and CHO negative control cell (Black).
Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
Ligand (PD-L1) working concentration: 1 μg/ml
The signal was developed with Alexa Fluor 647 Conjugated Affinipure Goat anti-human IgG (H + L).

Human PD-1 Antibody (PD1.H5), MAb, Mouse

Immunohistochemistry analysis of human tonsil tissue using Human PD-1 Antibody PD1.H5(GenScript, A01828)

Synonyms Mouse monoclonal to programmed cell death 1/ PD-1/ CD279/ SLEB2/ hPD-1/ hPD-l/ hSLE1.
Description Programmed cell death protein 1 (PD-1), is cell surface receptor expressing on T cells and pro-B cells. Binding of its two ligands PD-L1 and PD-L2 could result in down-regulation of the immune system by inhibiting the T-cell activation process. Thus, PD-1 is an important immune checkpoint and popular target for therapeutic antibodies against many cancers.
GenScript Anti-Human PD-1 Antibody (PD1.H5), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human PD-1-Fc (Z03370).
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Recombinant human PD-1-Fc (Z03370)
Purification Protein A affinity column

Subclass IgG1,κ
Clone ID PD1.H5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (This can be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human PD-1 (UniProt Accession: Q15116). This antibody blocks human PD-L1 binding with human PD-1 expressing cell line in flow cytometry assay and human mixed-lymphocyte reaction assay.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Flow cytometry: 2-10 µg/ml
Blockade of Receptor-ligand Interaction in Flow cytometry: 2-10 µg/ml
IHC: 5-10 µg/ml
Other applications: user-optimized


  • Human PD-1 Antibody (PD1.H5), MAb, Mouse
  • Human PD-1 Antibody (PD1.H5), MAb, Mouse

    ELISA binding of human PD-1 antibody PD1.H5 (GenScript, A01828) with Human PD-1 recombinant protein (Z03370, PD 1 Fc Chimera, Human).
    Coating antigen: PD-1-Fc, 1 µg/ml.
    PD-1 antibody dilution start from 1000 ng/ml, EC50= 4.29 ng/ml

  • Human PD-1 Antibody (PD1.H5), MAb, Mouse
  • Human PD-1 Antibody (PD1.H5), MAb, Mouse

    ELISA blocking of human PD-1 antibody PD1.H5 (GenScript, A01828) against Human PD-L1 recombinant protein (Z03371, PD 1 Fc Chimera, Human) binding with Human PD-1 recombinant protein (Z03370, PD 1 Fc Chimera, Human).
    Coating antigen: PD-1-Fc, 1 µg/ml.
    PD-L1-Fc final concentration: 0.5 µg /ml
    PD-1 antibody dilution start from 50 µg/ml, IC50= 15.5 µg/ml

  • Human PD-1 Antibody (PD1.H5), MAb, Mouse
  • Human PD-1 Antibody (PD1.H5), MAb, Mouse

    Flow cytometric analysis of CHO-K1/PD1 stable cell expressing PD-1 (GenScript, M00529, Red) and CHO negative control cell (Black) binding with Human PD-1 Antibody PD1.H5 (GenScript, A01828)
    Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

  • Human PD-1 Antibody (PD1.H5), MAb, Mouse
  • Human PD-1 Antibody (PD1.H5), MAb, Mouse

    FACS ligand blocking tests of Human PD-1 Antibody PD1.H5 (GenScript, A01828) blocks the binding of human PD-1 cell line (GenScript, M00529) with Human PD-L1 (Z03371, PD L1 Fc Chimera, Human. Red) and CHO negative control cell (Black).
    Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
    Ligand (PD-L1) working concentration: 1 μg/ml
    The signal was developed with Alexa Fluor 647 Conjugated Affinipure Goat anti-human IgG (H + L).

  • Human PD-1 Antibody (PD1.H5), MAb, Mouse
  • Human PD-1 Antibody (PD1.H5), MAb, Mouse

    Immunohistochemistry analysis of human tonsil tissue using Human PD-1 Antibody PD1.H5(GenScript, A01828)


Cat. No.
Product Name
Price
Z03370-1
PD-1 Fc Chimera, Human - 1 mg
$1200.00
Z03370-50
PD-1 Fc Chimera, Human - 50 ug
$120.00
 



Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01828
Size
Price
40 ug $99.00