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Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody Product » MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit

MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit

MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

MonoRab™ Anti-Pembrolizumab antibody (90G12) (GenScript, A01853-40) binds with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Pembrolizumab, 1 µg/ml.
Anti-Pembrolizumab antibody (GenScript, A01853-40) dilution start from 1,000 ng/ml.
EC50= 5.01 ng/ml.

MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

MonoRab™ Anti-Pembrolizumab antibody (90G12) (GenScript, A01853-40) blocks Pembrolizumab binding with Human PD-1 recombinant protein (Z03370, PD 1-Fc Chimera, Human).
Coating antigen: Pembrolizumab, 1 µg/ml.
PD 1-Fc-biotin final concentration: 20 ng/ml.
Anti-Pembrolizumab antibody (GenScript, A01853-40) dilution start from 10 µg/ml.
IC50= 0.38 μg/ml.

MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

Affinity of MonoRab Anti-Pembrolizumab Antibody (90G12), mAb, Rabbit (Cat.No. A01853) , Kd < 10⁻¹²M

Description Pembrolizumab (Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Pembrolizumab binds to the human cell surface receptor PD-1 (Programmed Cell Death Protein 1) and blocks its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells.
GenScript MonoRab™ Anti-Pembrolizumab Antibody (90G12)[BIOTIN], mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
Host Species Rabbit
Conjugation Biotin
Immunogen Pembrolizumab
Purification Protein A affinity column

Subclass IgG
Clone ID 90G12
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. (It could be customized for bulk order)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for Pembrolizumab. This antibody blocks Pembrolizumab binding with human PD-1 recombinant protein (Z03370, PD 1 Fc Chimera, Human) in ELISAs. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01852-40, Anti-Pembrolizumab Antibody (129G11B9), mAb, Rabbit.
Storage The lyophilized product remains stable up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
ELISA blocking: 10-50 µg/ml
Other applications: user-optimized

  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit
  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

    MonoRab™ Anti-Pembrolizumab antibody (90G12) (GenScript, A01853-40) binds with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
    Coating antigen: Pembrolizumab, 1 µg/ml.
    Anti-Pembrolizumab antibody (GenScript, A01853-40) dilution start from 1,000 ng/ml.
    EC50= 5.01 ng/ml.

  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit
  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

    MonoRab™ Anti-Pembrolizumab antibody (90G12) (GenScript, A01853-40) blocks Pembrolizumab binding with Human PD-1 recombinant protein (Z03370, PD 1-Fc Chimera, Human).
    Coating antigen: Pembrolizumab, 1 µg/ml.
    PD 1-Fc-biotin final concentration: 20 ng/ml.
    Anti-Pembrolizumab antibody (GenScript, A01853-40) dilution start from 10 µg/ml.
    IC50= 0.38 μg/ml.

  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit
  • MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], MAb, Rabbit

    Affinity of MonoRab Anti-Pembrolizumab Antibody (90G12), mAb, Rabbit (Cat.No. A01853) , Kd < 10⁻¹²M




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Cat. No. A01853
Size
Price
40 ug $198.00