|Synonyms||Rabbit monoclonal to Keytruda|
(Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food
and Drug Administration for the treatment of patients with unresectable or
metastatic melanoma. Pembrolizumab binds to the human cell surface receptor
PD-1 (Programmed Cell Death Protein 1) and blocks its interaction with PD-L1,
resulting in the activation of T-cell-mediated immune responses against tumor
GenScript MonoRab™ Anti-Pembrolizumab Antibody (90G12F8) [Biotin], mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized
with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order)
lyophilized antibody with deionized water (or equivalent) to a final
concentration of 0.5 mg/ml.
|Specificity||The product is specific for Pembrolizumab. This
antibody blocks Pembrolizumab binding with human PD-1 recombinant protein
(Z03370, PD 1 Fc Chimera, Human) in ELISAs.
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
concentrations for specific applications should be determined by the
investigators. The appropriate concentrations may be
affected by secondary antibody affinity, antigen concentration, the sensitivity
of the method of detection, temperature, the length of the incubations, and
other factors. The suitability of this antibody for applications other than
those listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Other applications: user-optimized
MonoRab™ Anti-Pembrolizumab antibody (90G12) (GenScript, A01853-40) binds with Pembrolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Pembrolizumab, 1 µg/ml.
Anti-Pembrolizumab antibody (GenScript, A01853-40) dilution start from 1,000 ng/ml.
EC50= 5.01 ng/ml.
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