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Catalog Products » Antibodies » Primary Antibodies » Immune Checkpoint Antibodies » Anti-Ipilimumab Antibody (4H6)[Biotin], mAb, Mouse

Anti-Ipilimumab Antibody (4H6)[Biotin], mAb, Mouse

Synonyms Mouse monoclonal to Yervoy
Description Ipilimumab (Yervoy) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Ipilimumab works to activate the immune system by targeting CTLA-4 (Cytotoxic T-lymphocyte protein 4), a negative regulator of cytotoxic T-lymphocyte activity. Ipilimumab binds to CTLA-4, blocking the inhibitory signal, which allows the cytotoxic T-lymphocytes to destroy the cancer cells
Anti-Ipilimumab Antibody (4H6) [biotin], mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Ipilimumab.
Host Species Mouse
Conjugation Biotin
Immunogen Ipilimumab
Purification Protein A affinity column

Subclass IgG1
Clone ID 4H6
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. (It could be customized for bulk order)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for Ipilimumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01859-40, Anti- Ipilimumab Antibody (26B6H7D9), mAb, Mouse.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Other applications: user-optimized

  • Anti-Ipilimumab Antibody (4H6)[Biotin], MAb, Mouse
  • Anti-Ipilimumab Antibody (4H6)[Biotin], MAb, Mouse

    Anti-Ipilimumab Antibody (4H6) [biotin], mAb, Mouse (GenScript, A01858-40) binds with Ipilimumab, while the antibody does not recognize human IgG Fc fragment (data not shown).
    Coating antigen: Ipilimumab, 1 µg/ml.
    Anti-Ipilimumab antibody (GenScript, A01858-40) dilution start from 1,000 ng/ml.
    EC₅₀=3.85 ng/ml.


Cat. No.
Product Name
Price
A01961-40
Anti-Ipilimumab Antibody (4H6), mAb, Mouse - 40 µg
$99.00
A01859-40
Anti-Ipilimumab Antibody(26B6H7D9), mAb, Mouse - 40 ug
$99.00
 



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Cat. No. A01858
Size
Price
40 ug $99.00