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Catalog Products » Antibodies » Primary Antibodies » Immune Checkpoint Antibodies » Human VISTA Antibody (VA.F6), mAb, Mouse

Human VISTA Antibody (VA.F6), mAb, Mouse

Human VISTA Antibody (VA.F6), MAb, Mouse

ELISA binding of human VISTA antibody VA.F6 (GenScript) with Human VISTA recombinant protein (genscript, VISTA Fc Chimera, Human).
Coating antigen: VISTA-Fc, 1 µg/ml.
VISTA antibody dilution start from 1000 ng/ml, EC₅₀=3.953 ng/ml

Human VISTA Antibody (VA.F6), MAb, Mouse

Flow cytometric analysis of CHO-K1/VISTA stable cell line (GenScript, M00533, Red) and CHO negative control cell (Black) binding with Human VISTA Antibody VA.F6 (GenScript, )
Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Synonyms Mouse monoclonal to VSIR/V-set immunoregulatory receptor/B7H5/ GI24/ B7-H5/ PD-1H/ SISP1/ PP2135/ C10orf54/ DD1alphaPDCD1 Homolog/V-Set Domain-Containing Immunoregulatory Receptor/V-Domain Ig Suppressor Of T Cell Activation / Chromosome 10 Open Reading Fr
Description VISTA is a negative immune checkpoint regulator that suppresses T-cell activation, and highly expressed on hematopoietic cells and within the tumor microenvironment. By analogy to VISTA and PD-L1 blockade, VISTA blockade may offer an immunotherapeutic strategy for human cancer. Therapeutic antibody against VISTA activates T cell signal and offers a way to promote this blockade.
GenScript Human VISTA Antibody (VA.F6), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with recombinant human VISTA.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Recombinant human VISTA-Fc
Purification Protein A affinity column

Subclass IgG2a,κ
Clone ID VA.F6
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. GenScript can customize this product per customer's request including product size, buffer components, etc.
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human VISTA (UniProt Accession: Q9H7M9; Gene ID: 64115). This antibody binds with recombinant VISTA protein in ELISA and human VISTA expressing cell line in flow cytometry assay.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.01-0.1 µg/ml
Flow cytometry: 2-10 µg/ml
Other applications: user-optimized

  • Human VISTA Antibody (VA.F6), MAb, Mouse
  • Human VISTA Antibody (VA.F6), MAb, Mouse

    ELISA binding of human VISTA antibody VA.F6 (GenScript) with Human VISTA recombinant protein (genscript, VISTA Fc Chimera, Human).
    Coating antigen: VISTA-Fc, 1 µg/ml.
    VISTA antibody dilution start from 1000 ng/ml, EC₅₀=3.953 ng/ml

  • Human VISTA Antibody (VA.F6), MAb, Mouse
  • Human VISTA Antibody (VA.F6), MAb, Mouse

    Flow cytometric analysis of CHO-K1/VISTA stable cell line (GenScript, M00533, Red) and CHO negative control cell (Black) binding with Human VISTA Antibody VA.F6 (GenScript, )
    Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG




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Cat. No. A01864
Size
Price
40 µg $99.00