MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) dilution start from 1,000 ng/ml.
EC50= 47.78 ng/ml.
Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) to DYKDDDDK-tag Fusion Protein by Western blot
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A: N-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
B: C-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
C: Internal positions DYKDDDDK-tagged fusion protein (50ng, 25 ng, 10 ng, 5 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml
Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40).
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Lane 1: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 2: Rabbit IgG (4 μg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input)
Lane 4: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 5: Rabbit IgG (4 μg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211) 0.125 μg/ml
MonoRab™ DYKDDDDK Tag Antibody, mAb, Rabbit
A01868 | |
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$205.00 | |
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