×
Catalog Products » Other Products » MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) dilution start from 1,000 ng/ml.
EC50= 47.78 ng/ml.

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) to DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng)
Lane 5: C-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 6: C-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 7: C-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 8: C-terminal DYKDDDDK-tagged fusion protein (1 ng)
Lane 9: Internal positions DYKDDDDK-tagged fusion protein (50 ng)
Lane 10: Internal positions DYKDDDDK-tagged fusion protein (25 ng)
Lane 11: Internal positions DYKDDDDK-tagged fusion protein (10 ng)
Lane 12: Internal positions DYKDDDDK-tagged fusion protein (5 ng)
Lane 13: Internal positions DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40).
Loading:
Lane 1: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 2: Rabbit IgG (4 μg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input)
Lane 4: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 5: Rabbit IgG (4 μg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211) 0.125 μg/ml

Synonyms Rabbit monoclonal to Flag
Description The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabTM technology to generate the high affinity and specificity monoclonal rabbit antibodies. GenScript MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Host Species Rabbit
Conjugation Unconjugated
Immunogen DYKDDDDK synthetic peptide coupled to KLH
Purification Protein A affinity column

Subclass IgG
Clone ID 114F12C8
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit specifically recognizes DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western Blot: 0.2-1 μg/ml
Immunoprecipitation (IP): 0.2-1 μg/ml
Other applications: user-optimized

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit
MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (GenScript, A01868-40) dilution start from 1,000 ng/ml.
EC50= 47.78 ng/ml.

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit
MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) to DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng)
Lane 5: C-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 6: C-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 7: C-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 8: C-terminal DYKDDDDK-tagged fusion protein (1 ng)
Lane 9: Internal positions DYKDDDDK-tagged fusion protein (50 ng)
Lane 10: Internal positions DYKDDDDK-tagged fusion protein (25 ng)
Lane 11: Internal positions DYKDDDDK-tagged fusion protein (10 ng)
Lane 12: Internal positions DYKDDDDK-tagged fusion protein (5 ng)
Lane 13: Internal positions DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit
MonoRabᵀᴹ DYKDDDDK Tag Antibody, MAb, Rabbit

Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40).
Loading:
Lane 1: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 2: Rabbit IgG (4 μg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein
Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input)
Lane 4: MonoRabTM DYKDDDDK antibody (4 μg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 5: Rabbit IgG (4 μg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein
Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit (A01868-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor, 926-32211) 0.125 μg/ml




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01868
Size
Price
40 µg $198.00
24 hour
 
*
*
*
*